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Literature summary for 1.3.5.1 extracted from
- Ubiquinone-binding site mutations in the Saccharomyces cerevisiae succinate dehydrogenase generate superoxide and lead to the accumulation of succinate (2007), J. Biol. Chem., 282, 27518-27526.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phenazine methosulfate | - |
Saccharomyces cerevisiae |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| genes SDHA, SDHB, SDHC, and SDHD | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D88E | site-directed mutagenesis of a subunit Sdh4p residue, the mutant shows reduced activity, as well as accumulation and secretion of succinate, the mutant is sensitive to hyperoxia and paraquate and shows enhanced superoxide production in vivo and in vitro | Saccharomyces cerevisiae |
| D88K | site-directed mutagenesis of a subunit Sdh4p residue, the mutant shows reduced activity and FAD content | Saccharomyces cerevisiae |
| D88N | site-directed mutagenesis of a subunit Sdh4p residue, the mutant shows reduced activity and FAD content, as well as accumulation and secretion of succinate, the mutant is sensitive to hyperoxia and paraquate and shows enhanced superoxide production in vivo and in vitro | Saccharomyces cerevisiae |
| additional information | ubiquinone-binding site mutations in the succinate dehydrogenase generate superoxide and lead to the accumulation of succinate | Saccharomyces cerevisiae |
| R47C | site-directed mutagenesis of a subunit Sdh3p residue, the mutant shows reduced activity | Saccharomyces cerevisiae |
| R47E | site-directed mutagenesis of a subunit Sdh3p residue, the mutant shows reduced activity and FAD content | Saccharomyces cerevisiae |
| R47K | site-directed mutagenesis of a subunit Sdh3p residue, the mutant shows reduced activity, as well as accumulation and secretion of succinate, the mutant is sensitive to hyperoxia and paraquate and shows enhanced superoxide production in vivo and in vitro | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| malonate | - |
Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrial membrane | - |
Saccharomyces cerevisiae | 31966 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| succinate + ubiquinone | Saccharomyces cerevisiae | SDH is an essential component of the electron transport chain and of the tricarboxylic acid cycle in the mitochondrial membrane | fumarate + ubiquinol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
genes SDHA, SDHB, SDHC, and SDHD encding the four subunits of the enzyme | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
substrate specificities of wild-type and mutant enzymes, overview | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| succinate + decylubiquinone | decylubiquinone-mediated reduction of dichlorophenol indophenol | Saccharomyces cerevisiae | fumarate + decylubiquinol | - |
? | |
| succinate + oxidized 2,6-dichlorophenolindophenol | succinate-dependent, phenazine methosulfate-mediated malonate-sensitive reduction of 2,6-dichlorophenol indophenol | Saccharomyces cerevisiae | fumarate + reduced 2,6-dichlorophenolindophenol | - |
? | |
| succinate + ubiquinone | SDH is an essential component of the electron transport chain and of the tricarboxylic acid cycle in the mitochondrial membrane | Saccharomyces cerevisiae | fumarate + ubiquinol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | subunits SdhA, SdhB, SdhC, and SdhD | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| mitochondrial succinate dehydrogenase | - |
Saccharomyces cerevisiae |
| SDH | - |
Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 2,6-dichlorophenolindophenol | - |
Saccharomyces cerevisiae | |
| decylubiquinone | - |
Saccharomyces cerevisiae | |
| FAD | - |
Saccharomyces cerevisiae | |
| ubiquinone | - |
Saccharomyces cerevisiae | |
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