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Literature summary for 1.3.5.1 extracted from
- Retention of heme in axial ligand mutants of succinate-ubiquinone xxidoreductase (complex II) from Escherichia coli (2001), J. Biol. Chem., 276, 18968-18976.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H71Q | mutation in SdhC subunit, 82% and 69% of wild-type kcat with succinate-phenazine ethosulfate and succinate, respectively | Escherichia coli |
| H84L | mutation in SdhC subunit, 54% and 23% of wild-type kcat with succinate-phenazine ethosulfate and succinate, respectively | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Pentachlorophenol | - |
Escherichia coli |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0025 | - |
succinate | 30°C, pH 7.8 | Escherichia coli | |
| 0.04 | - |
succinate | 30°C, pH 7.8, H84L mutant | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | [2Fe-2S], [4Fe-4S] and [3Fe-4S] clusters in SdhB subunit | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| succinate + ubiquinone | Escherichia coli | - |
fumarate + ubiquinol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant SQR | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| succinate + oxidized phenazine ethosulfate | - |
Escherichia coli | fumarate + reduced phenazine ethosulfate | - |
r | |
| succinate + phenazine ethosulfate | - |
Escherichia coli | fumarate + reduced phenazine ethosulfate | - |
? | |
| succinate + ubiquinone | - |
Escherichia coli | fumarate + ubiquinol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| complex II | - |
Escherichia coli |
| SQR | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 78 | - |
succinate | 30°C, pH 7.8 | Escherichia coli | |
| 82 | - |
oxidized phenazine ethosulfate | 30°C, pH 7.8 | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | flavoprotein subunit SdhA | Escherichia coli | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.017 | - |
Pentachlorophenol | 30°C, pH 7.8 | Escherichia coli | |
| 0.083 | - |
Pentachlorophenol | 30°C, pH 7.8, H84L mutant | Escherichia coli | |
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