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Literature summary for 1.3.5.1 extracted from
- Solubilization, purification, and characterization of succinate dehydrogenase from membranes of Mycobacterium phlei (1986), J. Bacteriol., 167, 1-6.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cardiolipin | stimulation in presence of detergent | Mycolicibacterium phlei |  |
| menaquinone-9(2H) | - |
Mycolicibacterium phlei | |
General Stability
| General Stability | Organism |
|---|---|
| stable during purification | Mycolicibacterium phlei |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fumarate | competitive | Mycolicibacterium phlei | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
succinate | - |
Mycolicibacterium phlei | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell membrane | - |
Mycolicibacterium phlei | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe-S-clusters | - |
Mycolicibacterium phlei | |
| Iron | - |
Mycolicibacterium phlei | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
? * 62000 + ? * 26000 | Mycolicibacterium phlei |
| 62000 | - |
? * 62000 + ? * 26000 | Mycolicibacterium phlei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycolicibacterium phlei | - |
succinate dehydrogenase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mycolicibacterium phlei |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 19 | - |
- |
Mycolicibacterium phlei |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C | Mycolicibacterium phlei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| succinate + electron acceptor | acceptor: phenazine methosulfate and 2,6-dichloroindophenol | Mycolicibacterium phlei | fumarate + reduced acceptor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | ? * 62000 + ? * 26000 | Mycolicibacterium phlei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Mycolicibacterium phlei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
- |
Mycolicibacterium phlei |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Mycolicibacterium phlei | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
fumarate | - |
Mycolicibacterium phlei | |
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