Literature summary for 1.3.5.1 extracted from

Burke, J.J.; Siedow, J.N.; Moreland, D.E.
Succinate dehydrogenase. A partial purification from mung bean hypocotyls and soybean cotyledons (1982), Plant Physiol., 70, 1577-1581.

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Inhibitors

Inhibitors	Comment	Organism	Structure
malonate	-	Glycine max
malonate	-	Vigna radiata
oxaloacetate	-	Glycine max
oxaloacetate	-	Vigna radiata

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	inner membrane	Glycine max	5739	-
mitochondrion	inner membrane	Vigna radiata	5739	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe	-	Glycine max
Fe	-	Vigna radiata

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
13000	-	1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE	Glycine max
13000	-	1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE	Vigna radiata
15000	-	1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE	Glycine max
15000	-	1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE	Vigna radiata
30000	-	1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE	Glycine max
30000	-	1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE	Vigna radiata
67000	-	1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE	Glycine max
67000	-	1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE	Vigna radiata

Organism

Organism	UniProt	Comment	Textmining
Glycine max	-	soybean, succinate dehydrogenase	-
Vigna radiata	-	mung bean, succinate dehydrogenase	-

Oxidation Stability

Oxidation Stability	Organism
O2, 22% loss of activity during solublization period, only 3% loss if solubilization is performed under Ar	Glycine max
O2, 22% loss of activity during solublization period, only 3% loss if solubilization is performed under Ar	Vigna radiata

Purification (Commentary)

Purification (Comment)	Organism
partial, complex II preparation, using Triton X-100 extraction followed by ammonium sulfate precipitation	Glycine max
partial, complex II preparation, using Triton X-100 extraction followed by ammonium sulfate precipitation	Vigna radiata

Source Tissue

Source Tissue	Comment	Organism	Textmining
cotyledon	-	Glycine max	-
hypocotyl	-	Vigna radiata	-

Storage Stability

Storage Stability	Organism
4°C, N2-atmosphere	Glycine max
4°C, N2-atmosphere	Vigna radiata

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
succinate + ubiquinone	-	Glycine max	fumarate + ubiquinol	-	?
succinate + ubiquinone	-	Vigna radiata	fumarate + ubiquinol	-	?

Subunits

Subunits	Comment	Organism
tetramer	1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE	Glycine max
tetramer	1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE	Vigna radiata

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
4	-	enzyme activity remains constant for up to 5 h	Glycine max
4	-	enzyme activity remains constant for up to 5 h	Vigna radiata
25	-	62% loss of activity within 1 h	Glycine max
25	-	62% loss of activity within 1 h	Vigna radiata

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	7.5	-	Glycine max
7	7.5	-	Vigna radiata

Cofactor

Cofactor	Comment	Organism	Structure
cytochrome b	-	Glycine max
cytochrome b	-	Vigna radiata
cytochrome b-560	-	Glycine max
cytochrome b-560	-	Vigna radiata
additional information	the enzyme contains iron-sulfur centers	Glycine max
additional information	the enzyme contains iron-sulfur centers	Vigna radiata

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00034	-	oxaloacetate	-	Vigna radiata
0.00115	-	oxaloacetate	-	Glycine max
0.0218	-	malonate	-	Vigna radiata
0.0246	-	malonate	-	Glycine max

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Release 2023.1 (January 2023)