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Literature summary for 1.3.5.1 extracted from
- Isolation and functional aspects of the fumarate reductase involved in the phosphorylative electron transport of Vibrio succinogenes (1980), Biochim. Biophys. Acta, 591, 275-288.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fumarate | - |
Wolinella succinogenes |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
2,3-dimethyl-1,4-naphthohydroquinone | - |
Wolinella succinogenes | |
| 0.35 | - |
fumarate | donor menadiol | Wolinella succinogenes | |
| 20 | - |
succinate | - |
Wolinella succinogenes | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell membrane | - |
Wolinella succinogenes | - |
- |
| cytoplasmic membrane | - |
Wolinella succinogenes | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | - |
Wolinella succinogenes | |
| Iron | - |
Wolinella succinogenes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
1 * 79000, FAD-binding subunit, 1 * 31000, Fe-S cluster containing subunit, 2 * 25000, cytochrome b containing subunits, SDS-PAGE | Wolinella succinogenes |
| 25000 | - |
enzyme containing cytochrome b, 1 * 79000 + 1 * 31000 + 1 * 25000 | Wolinella succinogenes |
| 31000 | - |
1 * 79000, FAD-binding subunit, 1 * 31000, Fe-S cluster containing subunit, 2 * 25000, cytochrome b containing subunits, SDS-PAGE | Wolinella succinogenes |
| 31000 | - |
enzyme containing cytochrome b, 1 * 79000 + 1 * 31000 + 1 * 25000 | Wolinella succinogenes |
| 31000 | - |
1 * 79000 + 1 * 31000, SDS-PAGE | Wolinella succinogenes |
| 79000 | - |
1 * 79000, FAD-binding subunit, 1 * 31000, Fe-S cluster containing subunit, 2 * 25000, cytochrome b containing subunits, SDS-PAGE | Wolinella succinogenes |
| 79000 | - |
enzyme containing cytochrome b, 1 * 79000 + 1 * 31000 + 1 * 25000 | Wolinella succinogenes |
| 79000 | - |
1 * 79000 + 1 * 31000, SDS-PAGE | Wolinella succinogenes |
| 110000 | 114000 | excluding cytochrome b, calculation from FAD-content and subunit composition | Wolinella succinogenes |
| 147000 | - |
calculation from Stokes radius and sedimentation coefficient | Wolinella succinogenes |
| 160000 | 167000 | including cytochrome b, calculation from FAD-content and subunit composition | Wolinella succinogenes |
| 200000 | - |
including cytochrome b, sedimentation equilibrium centrifugation | Wolinella succinogenes |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Wolinella succinogenes | - |
formerly Vibrio succinogenes, fumarate reductase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Wolinella succinogenes |
| using solubilization with Triton X-100, chromatography on hydroxyapatite column and DEAE-Sephadex column, the enzyme elutes from the ion-exchange column in two forms, one containing and the other lacking cytochrome b | Wolinella succinogenes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 140 | - |
- |
Wolinella succinogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fumarate + 2,3-dimethyl-1,4-naphthohydroquinone | cytochrome b-dependent in both directions | Wolinella succinogenes | succinate + 2,3-dimethyl-1,4-naphthoquinone | - |
r | |
| fumarate + anthrahydroquinonesulfonate | reaction catalyzed in the presence or absence of cytochrome b | Wolinella succinogenes | succinate + anthraquinonesulfonate | - |
? | |
| fumarate + electron donor | donor: anthrahydroquinonesulfonate | Wolinella succinogenes | succinate + oxidized donor | - |
r | |
| succinate + electron acceptor | acceptor: methylene blue (oxidized) | Wolinella succinogenes | fumarate + reduced acceptor | - |
r | |
| succinate + electron acceptor | acceptor: ferricyanide | Wolinella succinogenes | fumarate + reduced acceptor | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 79000 + 1 * 31000, SDS-PAGE | Wolinella succinogenes |
| tetramer | 1 * 79000, FAD-binding subunit, 1 * 31000, Fe-S cluster containing subunit, 2 * 25000, cytochrome b containing subunits, SDS-PAGE | Wolinella succinogenes |
| trimer | enzyme containing cytochrome b, 1 * 79000 + 1 * 31000 + 1 * 25000 | Wolinella succinogenes |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome b | 2 mol per mol FAD | Wolinella succinogenes | |
| cytochrome b | 2 mol per mol of FAD | Wolinella succinogenes | |
| FAD | - |
Wolinella succinogenes | |
| FAD | one protein-bound FAD linked to the 79000 Da peptide | Wolinella succinogenes | |
| additional information | the enzyme contains iron-sulfur centers | Wolinella succinogenes |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.35 | - |
fumarate | - |
Wolinella succinogenes | |
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