Protein Variants | Comment | Organism |
---|---|---|
additional information | identification of the rare naturally occuring Gly210 deletion in PPO from herbicide-resistant Amaranthus tuberculatus. this deletion does not affect the affinity of protoporphyrinogen IX nor the FAD content, but decreases the catalytic efficiency of the enzyme. The mutant shows a significant increase in the Kis for inhibitors and a switch in their interactions from competitive to mixed-type inhibition | Amaranthus tuberculatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acifluorfen | - |
Amaranthus tuberculatus | |
lactofen | - |
Amaranthus tuberculatus | |
MC-15608 | - |
Amaranthus tuberculatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
protoporphyrinogen IX | about, pH 7.4, temperature not specified in the publication | Amaranthus tuberculatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Amaranthus tuberculatus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protoporphyrinogen IX + 3 O2 | Amaranthus tuberculatus | - |
protoporphyrin IX + 3 H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amaranthus tuberculatus | Q0NZW5 | Gly210 deletion PPO mutant | - |
Amaranthus tuberculatus | Q0NZW6 | PPO wild-type | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
protoporphyrinogen IX + 3 O2 | - |
Amaranthus tuberculatus | protoporphyrin IX + 3 H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | Gly210 plays a key role in the alphaL helix-capping motif at the C-terminus of the alpha-8 helix which helps to stabilize the helix | Amaranthus tuberculatus |
Synonyms | Comment | Organism |
---|---|---|
PPO | - |
Amaranthus tuberculatus |
R-PPO | - |
Amaranthus tuberculatus |
S-PPO | - |
Amaranthus tuberculatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Amaranthus tuberculatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Amaranthus tuberculatus |
General Information | Comment | Organism |
---|---|---|
additional information | Gly210 plays a key role in the alphaL helix-capping motif at the C-terminus of the alpha-8 helix which helps to stabilize the helix, protein homology modelling, three-dimensional model, and molecular dynamics simulations of the mutant enzymes, overview | Amaranthus tuberculatus |
additional information | Gly210 plays a key role in the alphaL helix-capping motif at the C-terminus of the alpha-8 helix which helps to stabilize the helix, protein homology modelling, three-dimensional model, and molecular dynamics simulations of the wild-type enzyme, overview | Amaranthus tuberculatus |