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Literature summary for 1.3.3.4 extracted from
- A continuous fluorimetric assay for protoporphyrinogen oxidase by monitoring porphyrin accumulation (2005), Anal. Biochem., 344, 115-121.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | advantages of the continuous spectrofluorimetric assay over the discontinuous assay is of importance for both the kinetic characterization of recombinant PPOs and the detection of low concentrations of this enzyme in biological samples | Mus musculus |
| analysis | advantages of the continuous spectrofluorimetric assay over the discontinuous assay is of importance for both the kinetic characterization of recombinant PPOs and the detection of low concentrations of this enzyme in biological samples | Myxococcus xanthus |
| analysis | advantages of the continuous spectrofluorimetric assay over the discontinuous assay is of importance for both the kinetic characterization of recombinant PPOs and the detection of low concentrations of this enzyme in biological samples | Aquifex aeolicus |
| analysis | advantages of the continuous spectrofluorimetric assay over the discontinuous assay is of importance for both the kinetic characterization of recombinant PPOs and the detection of low concentrations of this enzyme in biological samples, may be useful for assessing diminished PPO activities in variegate porphyria patient samples | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli JM109 cells | Homo sapiens |
| overexpression in Escherichia coli JM109 cells | Myxococcus xanthus |
| overexpression in Escherichia coli JM109 cells | Aquifex aeolicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acifluorfen | - |
Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
protoporphyrinogen-IX | - |
Aquifex aeolicus | |
| 0.0036 | - |
protoporphyrinogen-IX | - |
Myxococcus xanthus | |
| 0.0038 | - |
protoporphyrinogen-IX | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Mus musculus | 5739 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aquifex aeolicus | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
| Myxococcus xanthus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by gel filtration | Homo sapiens |
| by gel filtration | Myxococcus xanthus |
| by gel filtration | Aquifex aeolicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0000007167 | - |
- |
Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protoporphyrinogen-IX + 3 O2 | - |
Mus musculus | protoporphyrin-IX + 3 H2O2 | - |
? | |
| protoporphyrinogen-IX + 3 O2 | - |
Homo sapiens | protoporphyrin-IX + 3 H2O2 | - |
? | |
| protoporphyrinogen-IX + 3 O2 | - |
Myxococcus xanthus | protoporphyrin-IX + 3 H2O2 | - |
? | |
| protoporphyrinogen-IX + 3 O2 | - |
Aquifex aeolicus | protoporphyrin-IX + 3 H2O2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PPO | - |
Mus musculus |
| PPO | - |
Homo sapiens |
| PPO | - |
Myxococcus xanthus |
| PPO | - |
Aquifex aeolicus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00053 | - |
acifluorfen | - |
Homo sapiens | |
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