Literature summary for 1.3.3.4 extracted from

Jeong, E.; Houn, T.; Kuk, Y.; Kim, E.S.; Chandru, H.K.; Baik, M.; Back, K.; Guh, J.O.; Han, O.
A point mutation of valine-311 to methionine in Bacillus subtilis protoporphyrinogen oxidase does not greatly increase resistance to the diphenyl ether herbicide oxyfluorfen (2003), Bioorg. Chem., 31, 389-397.

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Activating Compound

Activating Compound	Comment	Organism	Structure
imidazole	activates recombinant wild-type enzyme and mutant V311M	Bacillus subtilis
Tween 20	activates recombinant wild-type enzyme and mutant V311M	Bacillus subtilis

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type and mutant V311M as His-tagged enzymes in Escherichia coli strain BL21(DE3)	Bacillus subtilis

Protein Variants

Protein Variants	Comment	Organism
V311M	site-directed mutagenesis, mutant shows similar kinetics compared to the wild-type enzyme, but slightly decreased activity dependent on pH, and NaCl, Tween 20, and imidazole concentrations	Bacillus subtilis

Inhibitors

Inhibitors	Comment	Organism	Structure
NaCl	inhibits recombinant wild-type enzyme and mutant V311M	Bacillus subtilis
oxyfluorfen	a diphenyl ether herbicide, competitive, the mutant V311M is slightly less sensitive to inhibition than the wild-type enzyme, inhibition kinetics	Bacillus subtilis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0032	-	protoporphyrinogen-IX	recombinant wild-type enzyme and recombinant mutant V311M, pH 7.4, 25°C	Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
56000	-	x * 56000, recombinant wild-type enzyme and mutant V311M, SDS-PAGE	Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
protoporphyrinogen-IX + 3 O2	Bacillus subtilis	-	protoporphyrin-IX + 3 H2O2	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	P32397	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged wild-type and mutant V311M from Escherichia coli by nickel affinity chromatography	Bacillus subtilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
protoporphyrinogen-IX + 3 O2	-	Bacillus subtilis	protoporphyrin-IX + 3 H2O2	-	?

Subunits

Subunits	Comment	Organism
?	x * 56000, recombinant wild-type enzyme and mutant V311M, SDS-PAGE	Bacillus subtilis

Synonyms

Synonyms	Comment	Organism
Protox enzyme	-	Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
12.9	-	protoporphyrinogen-IX	recombinant mutant V311M, pH 7.4, 25°C	Bacillus subtilis
14.1	-	protoporphyrinogen-IX	recombinant wild-type enzyme, pH 7.4, 25°C	Bacillus subtilis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	recombinant wild-type enzyme	Bacillus subtilis
7.5	-	recombinant mutant V311M	Bacillus subtilis

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Bacillus subtilis

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.011	-	oxyfluorfen	recombinant wild-type enzyme, pH 7.4, 25°C	Bacillus subtilis
0.0177	-	oxyfluorfen	recombinant mutant V311M, pH 7.4, 25°C	Bacillus subtilis

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Release 2023.1 (January 2023)