Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-hydroxy-L-tryptophan | - |
Chromobacterium violaceum | |
D-tryptophan | - |
Chromobacterium violaceum | |
methyl-3-indole | - |
Chromobacterium violaceum | |
additional information | no inhibition with L-histidine, N-acetyl-L-phenylalaninamide and N-acetyl-L-tyrosinamide | Chromobacterium violaceum | |
tryptamine | - |
Chromobacterium violaceum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0195 | - |
N-Acetyl-L-tryptophanamide | - |
Chromobacterium violaceum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Chromobacterium violaceum | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14000 | - |
alpha, 4 * 14000 + beta 4 * 74000, organized in an alpha4,beta4 manner, heterooligomeric structure, one heme molecule per alpha,beta protomer, SDS-PAGE | Chromobacterium violaceum |
74000 | - |
alpha, 4 * 14000 + beta 4 * 74000, organized in an alpha4,beta4 manner, heterooligomeric structure, one heme molecule per alpha,beta protomer, SDS-PAGE | Chromobacterium violaceum |
680000 | - |
gel filtration | Chromobacterium violaceum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chromobacterium violaceum | - |
strain ATCC12472 | - |
Chromobacterium violaceum ATCC 12472 | - |
strain ATCC12472 | - |
Purification (Comment) | Organism |
---|---|
cell disruption with Eaton press, ultracentrifugation, ammonium sulfate precipitation, DEAE-column, concentration using centrifugal microcentrators, gel filtration, purified 108fold, yield 33.8% | Chromobacterium violaceum |
Storage Stability | Organism |
---|---|
-80°C, for several months and for 24-48 h at room temperature in the absence of any additives | Chromobacterium violaceum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-L-tryptophanamide + O2 | - |
Chromobacterium violaceum | N-acetyl-alpha,beta-didehydrotryptophanamide + H2O2 | - |
? | |
N-acetyl-L-tryptophanamide + O2 | - |
Chromobacterium violaceum ATCC 12472 | N-acetyl-alpha,beta-didehydrotryptophanamide + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | alpha, 4 * 14000 + beta 4 * 74000, organized in an alpha4,beta4 manner, heterooligomeric structure, one heme molecule per alpha,beta protomer, SDS-PAGE | Chromobacterium violaceum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Chromobacterium violaceum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.753 | - |
N-Acetyl-L-tryptophanamide | - |
Chromobacterium violaceum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 9.5 | - |
Chromobacterium violaceum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | NAD+, NADP+, FAD, or FMN show no effect on the activity | Chromobacterium violaceum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
methyl-3-indole | - |
Chromobacterium violaceum | |
0.0054 | - |
tryptamine | - |
Chromobacterium violaceum | |
0.0066 | - |
D-tryptophan | - |
Chromobacterium violaceum | |
0.037 | - |
5-hydroxy-L-tryptophan | - |
Chromobacterium violaceum |