General Stability | Organism |
---|---|
active with 0.2 M dithiothreitol, 1% SDS, or 4.5 M urea | Chromobacterium violaceum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-hydroxy-L-tryptophan | competitive inhibition, Ki: 0.037 mM | Chromobacterium violaceum | |
D-tryptophan | competitive inhibition, Ki: 0.0066 mM | Chromobacterium violaceum | |
methyl-3-indole | competitive inhibition, Ki: 0.0013 mM | Chromobacterium violaceum | |
additional information | no inhibition with L-histidine, N-acetyl-L-phenylalaninamide and N-acetyl-L-tyrosinamide | Chromobacterium violaceum | |
tryptamine | competitive inhibition, Ki: 0.0054 mMl | Chromobacterium violaceum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0058 | - |
L-tryptophan | - |
Chromobacterium violaceum | |
0.0091 | - |
L-Tryptophanamide | - |
Chromobacterium violaceum | |
0.0195 | - |
N-Acetyl-L-tryptophanamide | - |
Chromobacterium violaceum | |
0.02 | - |
indole-3-propionate | - |
Chromobacterium violaceum | |
0.026 | - |
pentagastrin | dehydrogenation of the tryptophanyl side chain | Chromobacterium violaceum | |
0.071 | - |
Luteinizing hormone-releasing hormone | dehydrogenation of the tryptophanyl side chain | Chromobacterium violaceum | |
0.0791 | - |
N-acetyl-L-tryptophan | - |
Chromobacterium violaceum | |
0.93 | - |
adrenocorticotropic hormone | dehydrogenation of the tryptophanyl side chain | Chromobacterium violaceum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
MgCl2 | 10 mM, residual activity 103% | Chromobacterium violaceum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14000 | - |
alpha, 4 * 14000 + beta 4 * 74000, organized in an alpha4,beta4 manner, heterooligomeric structure, one heme molecule per alpha,beta protomer, SDS-PAGE | Chromobacterium violaceum |
74000 | - |
alpha, 4 * 14000 + beta 4 * 74000, organized in an alpha4,beta4 manner, heterooligomeric structure, one heme molecule per alpha,beta protomer, SDS-PAGE | Chromobacterium violaceum |
680000 | - |
gel filtration | Chromobacterium violaceum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chromobacterium violaceum | - |
strain ATCC12472 | - |
Chromobacterium violaceum ATCC 12472 | - |
strain ATCC12472 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
30.3 | - |
after purification | Chromobacterium violaceum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adrenocorticotropic hormone + O2 | - |
Chromobacterium violaceum | ? + H2O2 | - |
? | |
indole-3-propionate + O2 | - |
Chromobacterium violaceum | ? + H2O2 | - |
? | |
indole-3-propionate + O2 | - |
Chromobacterium violaceum ATCC 12472 | ? + H2O2 | - |
? | |
L-tryptophan + O2 | - |
Chromobacterium violaceum | alpha,beta-didehydrotryptophan + H2O2 | - |
? | |
L-tryptophan + O2 | - |
Chromobacterium violaceum ATCC 12472 | alpha,beta-didehydrotryptophan + H2O2 | - |
? | |
L-tryptophanamide + O2 | - |
Chromobacterium violaceum | alpha,beta-didehydrotryptophanamide + H2O2 | - |
? | |
L-tryptophanamide + O2 | - |
Chromobacterium violaceum ATCC 12472 | alpha,beta-didehydrotryptophanamide + H2O2 | - |
? | |
luteinizing hormone-releasing hormone + O2 | - |
Chromobacterium violaceum | ? + H2O2 | - |
? | |
N-acetyl-L-tryptophan + O2 | - |
Chromobacterium violaceum | N-acetyl-alpha,beta-didehydrotryptophan + H2O2 | - |
? | |
N-acetyl-L-tryptophan + O2 | - |
Chromobacterium violaceum ATCC 12472 | N-acetyl-alpha,beta-didehydrotryptophan + H2O2 | - |
? | |
N-acetyl-L-tryptophanamide + O2 | - |
Chromobacterium violaceum | N-acetyl-alpha,beta-didehydrotryptophanamide + H2O2 | - |
? | |
N-acetyl-L-tryptophanamide + O2 | - |
Chromobacterium violaceum ATCC 12472 | N-acetyl-alpha,beta-didehydrotryptophanamide + H2O2 | - |
? | |
pentagastrin + O2 | - |
Chromobacterium violaceum | N-[(2E)-2-{[N-(tert-butoxycarbonyl)-beta-alanyl]amino}-3-(1H-indol-3-yl)prop-2-enoyl]-L-methionyl-L-alpha-aspartyl-L-phenylalaninamide + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | alpha, 4 * 14000 + beta 4 * 74000, organized in an alpha4,beta4 manner, heterooligomeric structure, one heme molecule per alpha,beta protomer, SDS-PAGE | Chromobacterium violaceum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
active up to 80øC | Chromobacterium violaceum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.283 | - |
adrenocorticotropic hormone | dehydrogenation of the tryptophanyl side chain | Chromobacterium violaceum | |
0.333 | - |
Luteinizing hormone-releasing hormone | dehydrogenation of the tryptophanyl side chain | Chromobacterium violaceum | |
0.533 | - |
pentagastrin | dehydrogenation of the tryptophanyl side chain | Chromobacterium violaceum | |
0.593 | - |
L-Tryptophanamide | - |
Chromobacterium violaceum | |
0.66 | - |
L-tryptophan | - |
Chromobacterium violaceum | |
0.753 | - |
N-Acetyl-L-tryptophanamide | - |
Chromobacterium violaceum | |
0.785 | - |
indole-3-propionate | - |
Chromobacterium violaceum | |
0.79 | - |
N-acetyl-L-tryptophan | - |
Chromobacterium violaceum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Chromobacterium violaceum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
after dilution in 0.1 M bis-Tris buffer | Chromobacterium violaceum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
methyl-3-indole | - |
Chromobacterium violaceum | |
0.0054 | - |
tryptamine | - |
Chromobacterium violaceum | |
0.0066 | - |
D-tryptophan | - |
Chromobacterium violaceum | |
0.037 | - |
5-hydroxy-L-tryptophan | - |
Chromobacterium violaceum |