Literature summary for 1.3.1.9 extracted from

Liu, N.; Cummings, J.E.; England, K.; Slayden, R.A.; Tonge, P.J.
Mechanism and inhibition of the FabI enoyl-ACP reductase from Burkholderia pseudomallei (2011), J. Antimicrob. Chemother., 66, 564-573.

Search for more literature for 1.3.1.9

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Burkholderia pseudomallei

Inhibitors

Inhibitors	Comment	Organism	Structure
2-phenoxy-5-propylphenol	minimal inhibitory concentration for Burkholderia pseudomallei growth above 250 mg/l	Burkholderia pseudomallei
5-ethyl-2-phenoxyphenol	minimal inhibitory concentration for Burkholderia pseudomallei growth 70 mg/l	Burkholderia pseudomallei
5-pentyl-2-phenoxyphenol	minimal inhibitory concentration for Burkholderia pseudomallei growth above 250 mg/l	Burkholderia pseudomallei
triclosan	minimal inhibitory concentration for Burkholderia pseudomallei growth 30 mg/l	Burkholderia pseudomallei

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0017	-	trans-2-dodecenoyl-CoA	pH 6.8, 25°C	Burkholderia pseudomallei
0.0056	-	trans-2-decenoyl-CoA	pH 6.8, 25°C	Burkholderia pseudomallei
0.027	-	crotonyl-[acyl-carrier-protein]	pH 6.8, 25°C	Burkholderia pseudomallei
0.16	-	trans-2-octenoyl-CoA	pH 6.8, 25°C	Burkholderia pseudomallei
0.188	-	crotonyl-CoA	pH 6.8, 25°C	Burkholderia pseudomallei

Organism

Organism	UniProt	Comment	Textmining
Burkholderia pseudomallei	Q3JQY0	-	-
Burkholderia pseudomallei 1710b	Q3JQY0	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
crotonyl-CoA + NADH + H+	-	Burkholderia pseudomallei	butyryl-CoA + NAD+	-	?
crotonyl-CoA + NADH + H+	-	Burkholderia pseudomallei 1710b	butyryl-CoA + NAD+	-	?
crotonyl-[acyl-carrier-protein] + NADH + H+	-	Burkholderia pseudomallei	butyryl-[acyl-carrier-protein] + NAD+	-	?
crotonyl-[acyl-carrier-protein] + NADH + H+	-	Burkholderia pseudomallei 1710b	butyryl-[acyl-carrier-protein] + NAD+	-	?
additional information	catalyzes the NADH-dependent reduction of 2-trans-dodecenoyl-CoA via a sequential Bi Bi mechanism	Burkholderia pseudomallei	?	-	?
additional information	catalyzes the NADH-dependent reduction of 2-trans-dodecenoyl-CoA via a sequential Bi Bi mechanism	Burkholderia pseudomallei 1710b	?	-	?
trans-2-decenoyl-CoA + NADH + H+	-	Burkholderia pseudomallei	decanoyl-CoA + NAD+	-	?
trans-2-decenoyl-CoA + NADH + H+	-	Burkholderia pseudomallei 1710b	decanoyl-CoA + NAD+	-	?
trans-2-dodecenoyl-CoA + NADH + H+	-	Burkholderia pseudomallei	dodecanoyl-CoA + NAD+	-	?
trans-2-dodecenoyl-CoA + NADH + H+	-	Burkholderia pseudomallei 1710b	dodecanoyl-CoA + NAD+	-	?
trans-2-octenoyl-CoA + NADH + H+	-	Burkholderia pseudomallei	octanoyl-CoA + NAD+	-	?

Synonyms

Synonyms	Comment	Organism
FabI-1	-	Burkholderia pseudomallei

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.6	-	crotonyl-CoA	pH 6.8, 25°C	Burkholderia pseudomallei
4	-	crotonyl-[acyl-carrier-protein]	pH 6.8, 25°C	Burkholderia pseudomallei
5.6	-	trans-2-decenoyl-CoA	pH 6.8, 25°C	Burkholderia pseudomallei
8.4	-	trans-2-dodecenoyl-CoA	pH 6.8, 25°C	Burkholderia pseudomallei
28.3	-	trans-2-octenoyl-CoA	pH 6.8, 25°C	Burkholderia pseudomallei

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.00000051	-	pH 6.8, 25°C	Burkholderia pseudomallei	5-ethyl-2-phenoxyphenol
0.0000013	-	pH 6.8, 25°C	Burkholderia pseudomallei	2-phenoxy-5-propylphenol
0.00000157	-	pH 6.8, 25°C	Burkholderia pseudomallei	triclosan
0.0000018	-	pH 6.8, 25°C	Burkholderia pseudomallei	5-pentyl-2-phenoxyphenol

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
20	-	crotonyl-CoA	pH 6.8, 25°C	Burkholderia pseudomallei
150	-	crotonyl-[acyl-carrier-protein]	pH 6.8, 25°C	Burkholderia pseudomallei
183	-	trans-2-octenoyl-CoA	pH 6.8, 25°C	Burkholderia pseudomallei
1000	-	trans-2-decenoyl-CoA	pH 6.8, 25°C	Burkholderia pseudomallei
5000	-	trans-2-dodecenoyl-CoA	pH 6.8, 25°C	Burkholderia pseudomallei

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)