Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Plasmodium falciparum |
Crystallization (Comment) | Organism |
---|---|
mutants A372M, K316A in cimolex with triclosan, to 2.5 and 2.05 A resolution, respectively. The enzyme has a conserved salt bridge which stabilizes the substrate binding loop and appears to be important for the active conformation | Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
A372M | mutation increases the affinity of the enzyme towards triclosan to almost double | Plasmodium falciparum |
A372V | mutation increases the affinity of the enzyme towards triclosan to almost double | Plasmodium falciparum |
A372V/K316A | enzymatically inactive | Plasmodium falciparum |
A372V/K316E | enzymatically inactive | Plasmodium falciparum |
D370A | enzymatically inactive | Plasmodium falciparum |
D370K | enzymatically inactive | Plasmodium falciparum |
K316A | no change in affinity to triclosan | Plasmodium falciparum |
K316E | enzymatically inactive | Plasmodium falciparum |
K316I | enzymatically inactive | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
triclosan | competitive with NADH, uncompetitive with NAD+ | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q9BJJ9 | - |
- |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000025 | - |
triclosan | wild-type, substrate NADH, pH not specified in the publication, temperature not specified in the publication | Plasmodium falciparum | |
0.000028 | - |
triclosan | wild-type, substrate NAD+, pH not specified in the publication, temperature not specified in the publication | Plasmodium falciparum |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000033 | - |
mutant A372V, pH not specified in the publication, temperature not specified in the publication | Plasmodium falciparum | triclosan | |
0.000035 | - |
mutant A372M, pH not specified in the publication, temperature not specified in the publication | Plasmodium falciparum | triclosan | |
0.000066 | - |
wild-type, pH not specified in the publication, temperature not specified in the publication | Plasmodium falciparum | triclosan | |
0.000066 | - |
mutant K316A, pH not specified in the publication, temperature not specified in the publication | Plasmodium falciparum | triclosan |