Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.1.9 extracted from

  • Saito, J.; Yamada, M.; Watanabe, T.; Iida, M.; Kitagawa, H.; Takahata, S.; Ozawa, T.; Takeuchi, Y.; Ohsawa, F.
    Crystal structure of enoyl-acyl carrier protein reductase (FabK) from Streptococcus pneumoniae reveals the binding mode of an inhibitor (2008), Protein Sci., 17, 691-699.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine targeting enoyl-ACP reductase is an attractive approach for the development of novel antibacterials Streptococcus pneumoniae

Cloned(Commentary)

Cloned (Comment) Organism
-
Streptococcus pneumoniae

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with 1, hanging drop vapour diffusion method, with 0.1 M MES, pH 5.5-7.0, 0.1 M NH4Cl, 0.2 M CaCl2, 8-15% 2-methyl-2,4-pentanediol , 8-15% polyethyleneglycol 1000, and 5 mM dithiothreitol Streptococcus pneumoniae
the crystal structure of enoyl-acyl carrier protein reductase is determined at 1.7 A, in complex with a phenylimidazole derivative inhibitor at 2.3 A Streptococcus pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
AG205
-
Streptococcus pneumoniae
methyl 2-[[(1H-benzimidazol-2-ylmethyl)carbamoyl]amino]-1,3-benzothiazole-6-carboxylate AG205 Streptococcus pneumoniae
NADH
-
Streptococcus pneumoniae
sodium (4-[2-[([[5-(pyridin-2-ylsulfanyl)-1,3-thiazol-2-yl]carbamoyl]amino)methyl]-1H-imidazol-4-yl]phenoxy)acetate competitive inhibitor with respect to NADH and uncompetitive inhibitor with respect to crotonyl-CoA Streptococcus pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
crotonyl-CoA
-
Streptococcus pneumoniae
0.067
-
NADH
-
Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Streptococcus pneumoniae FabK catalyzes the reduction of enoyl-ACPs with the concomitant oxidation of NADH ?
-
?

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae
-
-
-
Streptococcus pneumoniae Q8DR17
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
crotonyl-CoA + NAD+ inhibition assay Streptococcus pneumoniae ?
-
?
crotonyl-CoA + NADH + H+
-
Streptococcus pneumoniae butyryl-CoA + NAD+
-
?
additional information FabK catalyzes the reduction of enoyl-ACPs with the concomitant oxidation of NADH Streptococcus pneumoniae ?
-
?

Subunits

Subunits Comment Organism
dimer x-ray crystallography Streptococcus pneumoniae

Synonyms

Synonyms Comment Organism
enoyl-acyl carrier protein reductase
-
Streptococcus pneumoniae
FabK
-
Streptococcus pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
inhibition assay Streptococcus pneumoniae

Cofactor

Cofactor Comment Organism Structure
FMN
-
Streptococcus pneumoniae
NADH
-
Streptococcus pneumoniae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000011
-
sodium (4-[2-[([[5-(pyridin-2-ylsulfanyl)-1,3-thiazol-2-yl]carbamoyl]amino)methyl]-1H-imidazol-4-yl]phenoxy)acetate
-
Streptococcus pneumoniae
0.000029
-
NADH
-
Streptococcus pneumoniae