Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.1.9 extracted from

  • Muench, S.P.; Prigge, S.T.; Zhu, L.; Kirisits, M.J.; Roberts, C.W.; Wernimont, S.; McLeod, R.; Rice, D.W.
    Expression, purification and preliminary crystallographic analysis of the Toxoplasma gondii enoyl reductase (2006), Acta Crystallogr. Sect. F, F62, 604-606.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.9

Application

Application Comment Organism
medicine antibacterial target Toxoplasma gondii

Cloned(Commentary)

Cloned (Comment) Organism
into the pMALc2x vector without signal peptide coding sequence, a second construct contains in addition the sequence of the TEV protease cleaving site followed by a hexahistidine tag Toxoplasma gondii

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals of enoyl reductase in complex with NAD+ and triclosan, data are collected to beyond 2.6 A Toxoplasma gondii

Inhibitors

Inhibitors Comment Organism Structure
triclosan
-
 Toxoplasma gondii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-[acyl-carrier protein] + NAD+ Toxoplasma gondii
-
 trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Toxoplasma gondii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using a HiTrap Chelating HP and an amylose column Toxoplasma gondii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-[acyl-carrier protein] + NAD+
-
 Toxoplasma gondii trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
enoyl reductase
-
 Toxoplasma gondii
enoyl-[acyl-carrier-protein] reductase
-
 Toxoplasma gondii
ENR
-
 Toxoplasma gondii

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Toxoplasma gondii