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Literature summary for 1.3.1.9 extracted from

  • Lee, I.H.; Kim, E.J.; Cho, Y.H.; Lee, J.K.
    Characterization of a novel enoyl-acyl carrier protein reductase of diazaborine-resistant Rhodobacter sphaeroides mutant (2002), Biochem. Biophys. Res. Commun., 299, 621-627.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Cereibacter sphaeroides

Protein Variants

Protein Variants Comment Organism
G95S marginal increase in resistance to diazaborine Cereibacter sphaeroides
P155Q strong increase in resistance to diazaborine Cereibacter sphaeroides

Inhibitors

Inhibitors Comment Organism Structure
diazaborine 0.019 mM, 50% inhibition of wild-type FabI1, 0.25 mM, 50% inhibition of P155Q mutant FabI1 Cereibacter sphaeroides
triclosan 0.01 mM, 50% inhibition of wild-type FabI1, 0.15 mM, 50% inhibition of P155Q mutant FabI1 Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
NADH 30°C, pH 6.5 Cereibacter sphaeroides
1.6
-
trans-but-2-enoyl-[acyl-carrier protein] 30°C, pH 6.5 Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant FabI1 Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trans-but-2-enoyl-[acyl-carrier protein] + NADH
-
Cereibacter sphaeroides butanoyl-[acyl-carrier protein] + NAD+
-
?

Synonyms

Synonyms Comment Organism
enoyl-acyl carrier protein reductase
-
Cereibacter sphaeroides
FabI1
-
Cereibacter sphaeroides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.48
-
trans-but-2-enoyl-[acyl-carrier protein] 30°C, pH 6.5, G95S mutant Cereibacter sphaeroides
0.65
-
trans-but-2-enoyl-[acyl-carrier protein] 30°C, pH 6.5 Cereibacter sphaeroides
1
-
trans-but-2-enoyl-[acyl-carrier protein] 30°C, pH 6.5, P155Q mutant Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
NADH
-
Cereibacter sphaeroides

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.002
-
triclosane 30°C, pH 6.5 Cereibacter sphaeroides
0.008
-
triclosane 30°C, pH 6.5, G95S mutant Cereibacter sphaeroides
0.0084
-
diazaborine 30°C, pH 6.5 Cereibacter sphaeroides
0.03
-
diazaborine 30°C, pH 6.5, G95S mutant Cereibacter sphaeroides
0.18
-
triclosane 30°C, pH 6.5, P155Q mutant Cereibacter sphaeroides
0.29
-
diazaborine 30°C, pH 6.5, P155Q mutant Cereibacter sphaeroides