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Literature summary for 1.3.1.31 extracted from

  • Gao, X.; Ren, J.; Wu, Q.; Zhu, D.
    Biochemical characterization and substrate profiling of a new NADH-dependent enoate reductase from Lactobacillus casei (2012), Enzyme Microb. Technol., 51, 26-34.
    View publication on PubMed
Search for more literature for 1.3.1.31

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Lacticaseibacillus casei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.034
-
 NADH pH 7.0, 30°C Lacticaseibacillus casei
1.94
-
 2-cyclohexen-1-one pH 7.0, 30°C Lacticaseibacillus casei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42300
-
 calculated from cDNA Lacticaseibacillus casei
46800
-
 SDS-PAGE Lacticaseibacillus casei
170000
-
 gel filtration Lacticaseibacillus casei

Organism

Organism UniProt Comment Textmining
Lacticaseibacillus casei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using ammonium sulfate precipitation and treatment in an acidic buffer Lacticaseibacillus casei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6Z)-nona-2,6-dienal + NADH + H+ relative activity: 62.7% Lacticaseibacillus casei ?
-
 ?
(E)-2-decenal + NADH + H+ relative activity: 90% Lacticaseibacillus casei decanal + NAD+
-
 ?
(E)-2-hexen-1-al + NADH + H+ relative activity: 100% Lacticaseibacillus casei hexanal + NAD+
-
 ?
(E)-2-methyl-2-butenal + NADH + H+ relative activity: 59.2% Lacticaseibacillus casei 2-methylbutanal + NAD+
-
 ?
(E)-2-nonenal + NADH + H+ relative activity: 57% Lacticaseibacillus casei nonanal + NAD+
-
 ?
(E)-2-octenal + NADH + H+ relative activity: 75.4% Lacticaseibacillus casei octanal + NAD+
-
 ?
(E)-2-pentenal + NADH + H+ relative activity: 45% Lacticaseibacillus casei 2-pentanone + NAD+
-
 ?
(E)-3-nonen-2-one + NADH + H+ relative activity: 36.6% Lacticaseibacillus casei nonan-2-one + NAD+
-
 ?
(R)-(-)-carvone + NADH + H+ relative activity: 70% Lacticaseibacillus casei ?
-
 ?
2-cyclohexen-1-one + NADH + H+ relative activity: 830% Lacticaseibacillus casei cyclohexanone + NAD+
-
 ?
2-cyclohexen-1-one + NADH + H+
-
 Lacticaseibacillus casei 2-cyclohexanone + NAD+
-
 ?
2-isopropyl-5-methyl-2-hexenal + NADH + H+ relative activity: 15.5% Lacticaseibacillus casei 2-isopropyl-5-methylhexanal + NAD+
-
 ?
2-methyl-2-cyclopenten-1-one + NADH + H+ relative activity: 62.7% Lacticaseibacillus casei ?
-
 ?
3-methyl-2-cyclopentenone + NADH + H+ relative activity: 31% Lacticaseibacillus casei ?
-
 ?
bromomaleic anhydride + NADH + H+ relative activity: 107% Lacticaseibacillus casei ?
-
 ?
phenylmaleic anhydride + NADH + H+ relative activity: 402% Lacticaseibacillus casei ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 42300, gel filtration Lacticaseibacillus casei

Synonyms

Synonyms Comment Organism
enoate reductase
-
 Lacticaseibacillus casei
LacER
-
 Lacticaseibacillus casei

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 55 temp. optimum Lacticaseibacillus casei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 no activity after 1 h Lacticaseibacillus casei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3200
-
 NADH pH 7.0, 30°C Lacticaseibacillus casei
8400
-
 2-cyclohexen-1-one pH 7.0, 30°C Lacticaseibacillus casei

pH Range

pH Minimum pH Maximum Comment Organism
8 9 optimal pH range Lacticaseibacillus casei

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Lacticaseibacillus casei