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Literature summary for 1.3.1.31 extracted from

  • Liu, Y.J.; Pei, X.Q.; Lin, H.; Gai, P.; Liu, Y.C.; Wu, Z.L.
    Asymmetric bioreduction of activated alkenes by a novel isolate of Achromobacter species producing enoate reductase (2012), Appl. Microbiol. Biotechnol., 95, 635-645.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Achromobacter sp.

Organism

Organism UniProt Comment Textmining
Achromobacter sp. I3V5V5 JA81
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E)-2-cyano-3-phenylprop-2-enoic acid + NADPH conversion rate measured using HPLC after 48 h. Conversion: 83%, ee: racemic Achromobacter sp. 2-cyano-3-phenylpropanoic acid + NADP+
-
?
(2Z)-3-(4-chlorophenyl)-3-cyanoprop-2-enoic acid + NADPH conversion rate measured using HPLC after 48 h. Conversion: 80%, ee: 94% (R) Achromobacter sp. (R)-3-(4-chlorophenyl)-3-cyano-propanoic acid + NADP+
-
?
(Z)-3-cyano-3-phenyl-propenoic acid + NADPH + H+ highest enantiomeric excess of 98% ee is achieved at a glucose concentration of 1% (w/v) with a 47% conversion for an 18 h reaction. Concentration of 2-propanol also affects the activity and selectivity of the reaction, and a concentration of 5% (v/v) results in the best substrate conversion and ee value of product. Conversion rate measured using HPLC after 48 h. Conversion: 80%, ee: 98% (R) Achromobacter sp. (R)-3-cyano-3-phenylpropanoic acid + NADP+
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?
3-methyl-1-phenyl-1H-pyrrole-2,5-dione + NADPH conversion rate measured using HPLC after 48 h. Conversion: 99%, ee: 99% (R) Achromobacter sp. (R)-N-phenyl-2-methylsuccinimide + NADP+
-
?
ethyl (2Z)-3-nitro-2-phenylprop-2-enoate + NADPH conversion rate measured using HPLC after 48 h. Conversion: 40%, ee: 45% (R) Achromobacter sp. ethyl (2R)-3-nitro-2-phenylpropanoate + NADP+
-
?
[(1E)-1-nitroprop-1-en-2-yl]benzene + NADPH conversion rate measured using HPLC after 48 h. Conversion: 87%, ee: 97% (S) Achromobacter sp. (S)-1-nitro-2-phenylpropane + NADP+
-
?
[(1E)-2-nitroprop-1-en-1-yl]benzene + NADPH conversion rate measured using HPLC after 48 h. Conversion: 60%, ee: racemic Achromobacter sp. 1-phenyl-2-nitropropane + NADP+
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?

Synonyms

Synonyms Comment Organism
Achr-OYE3
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Achromobacter sp.
enoate reductase
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Achromobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
6
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conversion rate drops to 20% at pH 6.0 Achromobacter sp.
7
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highest conversion rate 54%, pH optimum Achromobacter sp.
8
-
48% conversion rate Achromobacter sp.