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Literature summary for 1.21.3.1 extracted from

  • Brown, C.D.; Neidig, M.L.; Neibergall, M.B.; Lipscomb, J.D.; Solomon, E.I.
    VTVH-MCD and DFT studies of thiolate bonding to [FeNO]7/[FeO2]8 complexes of isopenicillin N synthase: substrate determination of oxidase versus oxygenase activity in non-heme Fe enzymes (2007), J. Am. Chem. Soc., 129, 7427-7438.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.21.3.1

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli K12 strain JM109 in inclusion bodies Streptomyces microflavus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ IPNS is a unique mononuclear non-heme Fe enzyme, the enzyme contains [FeNO]7/[FeO2]8 complexes, combination of spectroscopic techniques including EPR, absorbance, circular dichroism, magnetic CD, and variable temperature, variable-field MCD studies of thiolate bonding and the cofactor electronic structure, computational methods and modeling, overview Streptomyces microflavus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 Streptomyces microflavus
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 isopenicillin + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces microflavus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant, solubilized enzyme Streptomyces microflavus

Renatured (Commentary)

Renatured (Comment) Organism
dissolution of the recombinant enzyme from Escherichia coli inclusion bodies with 7 M urea containing 20 mM DTT, 30 mM glycine, pH 10.0, requires vigorous stirring for over 2 hours at room temperature Streptomyces microflavus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2
-
 Streptomyces microflavus isopenicillin + H2O
-
 ?
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 the enzyme catalyzes the four electron oxidative double ring closure of its substrate Streptomyces microflavus isopenicillin + H2O
-
 ?

Synonyms

Synonyms Comment Organism
IPNS
-
 Streptomyces microflavus