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Literature summary for 1.21.3.1 extracted from

  • Palissa, H.; Von Doehren, H.; Kleinkauf, H.; Ting, H.H.; Baldwin, J.E.
    beta-Lactam biosynthesis in a Gram-negative eubacterium: purification and characterization of isopenicillin N synthase from Flavobacterium sp. strain SC 12.154 (1989), J. Bacteriol., 171, 5720-5728.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.21.3.1

Activating Compound

Activating Compound Comment Organism Structure
ascorbic acid 5fold stimulation Flavobacterium sp.
dithioerythritol absolutely required, highly stimulating Flavobacterium sp.
O2 required Flavobacterium sp.
polyethylene glycol 1500 20% stimulation up to concentration of 5% Flavobacterium sp.
Triton X-100 leads to 50% stimulation at concentration of 0.01% Flavobacterium sp.

Application

Application Comment Organism
pharmacology model system for study of endogenous functions of beta-lactams in bacteria Flavobacterium sp.

General Stability

General Stability Organism
unstable to 20 mM dithiothreitol during storage at -20°C, 10% activity remaining Flavobacterium sp.

Inhibitors

Inhibitors Comment Organism Structure
Co2+ moderate inhibition Flavobacterium sp.
cystamine 100% inhibition at 0.5 mM Flavobacterium sp.
cysteamine 43% inhibition at 1.5 mM Flavobacterium sp.
cysteine 60% inhibition at 1 mM Flavobacterium sp.
cystine 81% inhibition at 1.5 mM Flavobacterium sp.
GSH 70% inhibition at 1 mM Flavobacterium sp.
Mn2+ moderate inhibition Flavobacterium sp.
additional information ATP, NADPH and FAD: no effect; cations Na+, K+, Mg2+, Ca2+, Fe3+ do not affect the activity Flavobacterium sp.
Ni2+ moderate inhibition Flavobacterium sp.
Triton X-100 inhibits at concentration of 0.5% Flavobacterium sp.
Zn2+ moderate inhibition Flavobacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine
-
 Flavobacterium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Flavobacterium sp. 5829
-
membrane inactive associated form Flavobacterium sp. 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ stimulates Flavobacterium sp.
Fe2+ low concentration Flavobacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36500 38000 gel filtration and SDS-PAGE Flavobacterium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 Flavobacterium sp. common step in the biosynthesis of penicillins, cephalosporins and cephamycins isopenicillin N + 2 H2O product has antibiotic activity ?
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 Flavobacterium sp. SC 12.154 common step in the biosynthesis of penicillins, cephalosporins and cephamycins isopenicillin N + 2 H2O product has antibiotic activity ?

Organism

Organism UniProt Comment Textmining
Flavobacterium sp.
-
-
-
Flavobacterium sp. SC 12.154
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Flavobacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00129
-
 about, purified enzyme Flavobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine + O2
-
 Flavobacterium sp. isopenicillin N + H2O
-
 r
delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine + O2
-
 Flavobacterium sp. SC 12.154 isopenicillin N + H2O
-
 r
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2
-
 Flavobacterium sp. isopenicillin N + 2 H2O
-
 ?
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 common step in the biosynthesis of penicillins, cephalosporins and cephamycins Flavobacterium sp. isopenicillin N + 2 H2O product has antibiotic activity ?
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2
-
 Flavobacterium sp. SC 12.154 isopenicillin N + 2 H2O
-
 ?
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 common step in the biosynthesis of penicillins, cephalosporins and cephamycins Flavobacterium sp. SC 12.154 isopenicillin N + 2 H2O product has antibiotic activity ?

Subunits

Subunits Comment Organism
monomer 1 * 36500-38000, SDS-PAGE and gel filtration Flavobacterium sp.

Synonyms

Synonyms Comment Organism
IPNS
-
 Flavobacterium sp.
isopenicillin N-synthase
-
 Flavobacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
15 20
-
 Flavobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
 Flavobacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 9 30% activity remaining above pH 9.0, highly reduced activity below pH 6.0 Flavobacterium sp.