Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.20.4.1 extracted from

  • Sundaram, S.; Rathinasabapathi, B.; Ma, L.Q.; Rosen, B.P.
    An arsenate-activated glutaredoxin from the arsenic hyperaccumulator fern Pteris vittata L. regulates intracellular arsenite (2008), J. Biol. Chem., 283, 6095-6101.
    View publication on PubMed
Search for more literature for 1.20.4.1

Activating Compound

Activating Compound Comment Organism Structure
arsenate 10 mM increases enzyme activity by 1.6fold Pteris vittata

Application

Application Comment Organism
degradation PvGrx5 has a role in regulating intracellular arsenite levels, by either directly or indirectly modulating the aquaglyceroporin Pteris vittata

Cloned(Commentary)

Cloned (Comment) Organism
gene Pv5-6 encoding PvGrx5 expressed in Escherichia coli strain XL-1 Blue. PvGrx5 expressed in Escherichia coli mutants in which the arsenic resistance genes of the ars operon are deleted (strain AW3110), a deletion of the gene for the ArsC arsenate reductase (strain WC3110), and a strain in which the ars operon is deleted and the gene for the GlpF aquaglyceroporin is disrupted (strain OSBR1) Pteris vittata

Protein Variants

Protein Variants Comment Organism
C67A loss of both GRX activity and arsenic resistance Pteris vittata

Organism

Organism UniProt Comment Textmining
Pteris vittata Q1A5X4
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity purified Pteris vittata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arsenate + reduced glutaredoxin
-
 Pteris vittata arsenite + oxidized glutaredoxin
-
 ?

Synonyms

Synonyms Comment Organism
glutaredoxin
-
 Pteris vittata
Grx
-
 Pteris vittata
PvGRX5
-
 Pteris vittata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pteris vittata