Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect of CoA | Pyrococcus furiosus |
General Stability | Organism |
---|---|
10% glycerol and 2 mM DTT stabilize during purification | Pyrococcus furiosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arsenite | 50% inhibition at 1 mM | Pyrococcus furiosus | |
crotonaldehyde | substrate inhibition above 0.2 mM | Pyrococcus furiosus | |
cyanide | 50% inhibition at 8 mM | Pyrococcus furiosus | |
iodoacetate | 50% inhibition at 0.2 mM | Pyrococcus furiosus | |
additional information | no effect of CoA | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
crotonaldehyde | pH 8.4, 80°C, with methyl viologen as electron acceptor | Pyrococcus furiosus | |
1 | - |
glyceraldehyde | pH 8.4, 65°C, with methyl viologen as electron acceptor | Pyrococcus furiosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 6.6 gatoms per 85000g of protein | Pyrococcus furiosus | |
Molybdenum | part of a tungsten-molybdopterin cofactor | Pyrococcus furiosus | |
Tungsten | part of a tungsten-molybdopterin cofactor | Pyrococcus furiosus | |
Tungsten | 1.0 gatoms per 85000g of protein | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
1 * 80000, SDS-PAGE | Pyrococcus furiosus |
90000 | - |
gel filtration | Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
an aldehyde + H2O + oxidized ferredoxin | Pyrococcus furiosus | - |
an acid + H+ + reduced ferredoxin | - |
? | |
glyceraldehyde + H2O + oxidized ferredoxin | Pyrococcus furiosus | involved in glycolysis | glycerate + H+ + reduced ferredoxin | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
hyperthermophilic archaeon | - |
Pyrococcus furiosus | - |
strain DSM 3638 | - |
Oxidation Stability | Organism |
---|---|
sensitive against O2, dithionite is added to avoid trace contamination with O2 during purification and enzyme assay | Pyrococcus furiosus |
Purification (Comment) | Organism |
---|---|
to homogeneity under strict anaerobic conditions | Pyrococcus furiosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
53.6 | - |
purified enzyme, substrate crotonaldehyde with methyl viologen as electron acceptor | Pyrococcus furiosus |
Storage Stability | Organism |
---|---|
-196°C, pure enzyme pellet is thawed anaerobically after storage in liquid N2, no loss of activity for several months | Pyrococcus furiosus |
4°c or 23°C, pure enzyme, 50 mM Tris-HCl, pH 8.0, 2 mM sodium dithionite, 2 mM DTT, 10% v/v glycerol, loss of 25% activity after 6 h under strict anaerobic conditions | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + H2O + oxidized ferredoxin | - |
Pyrococcus furiosus | acetate + H+ + reduced ferredoxin | - |
ir | |
an aldehyde + H2O + oxidized ferredoxin | - |
Pyrococcus furiosus | an acid + H+ + reduced ferredoxin | - |
? | |
an aldehyde + H2O + oxidized ferredoxin | - |
Pyrococcus furiosus | an acid + H+ + reduced ferredoxin | - |
ir | |
butyraldehyde + H2O + oxidized ferredoxin | - |
Pyrococcus furiosus | butyrate + H+ + reduced ferredoxin | - |
ir | |
crotonaldehyde + H2O + oxidized ferredoxin | best substrate | Pyrococcus furiosus | crotonate + H+ + reduced ferredoxin | - |
ir | |
crotonaldehyde + H2O + oxidized methyl viologen | artifical electron acceptor | Pyrococcus furiosus | crotonate + H+ + reduced methyl viologen | - |
ir | |
formaldehyde + H2O + oxidized ferredoxin | - |
Pyrococcus furiosus | formate + H+ + reduced ferredoxin | - |
ir | |
glyceraldehyde + H2O + oxidized ferredoxin | - |
Pyrococcus furiosus | glycerate + H+ + reduced ferredoxin | - |
ir | |
glyceraldehyde + H2O + oxidized ferredoxin | involved in glycolysis | Pyrococcus furiosus | glycerate + H+ + reduced ferredoxin | - |
ir | |
glyceraldehyde + H2O + oxidized methyl viologen | - |
Pyrococcus furiosus | glycerate + H+ + reduced methyl viologen | - |
ir | |
additional information | enzyme shows an active and an inactive form | Pyrococcus furiosus | ? | - |
? | |
additional information | no oxidizing activity with glyceraldehyde-3-phosphate, glyoxylate, glucose, glucose 6-phosphate, CO, H2, formate, pyruvate, 2-oxoglutarate | Pyrococcus furiosus | ? | - |
? | |
additional information | NAD(P) is no electron acceptor | Pyrococcus furiosus | ? | - |
? | |
additional information | no H2 evolution from reduced methyl viologen | Pyrococcus furiosus | ? | - |
? | |
additional information | no activity with CoA | Pyrococcus furiosus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 80000, SDS-PAGE | Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
above | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme is thermostable | Pyrococcus furiosus |
23 | - |
pure enzyme, 5.2 mg/ml, 50 mM Tris-HCl, pH 8.0, 2 mM sodium dithionite, 2 mM DTT, 10% v/v glycerol, exposure to air, less than 20% remaining activity after 5 min, after 10 additional min 10% residual activity is reached, which stays constant for more than 30 min | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
sharp maximum | Pyrococcus furiosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.4 | activity increases with increasing pH at increasing temperature, nearly no activity below pH 7.0 | Pyrococcus furiosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | - |
Pyrococcus furiosus | |
tungsten-molybdopterin | tungstopterin center | Pyrococcus furiosus |