Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.4.1 extracted from

  • Kale, S.; Arjunan, P.; Furey, W.; Jordan, F.
    A dynamic loop at the active center of the Escherichia coli pyruvate dehydrogenase complex E1 component modulates substrate utilization and chemical communication with the E2 component (2007), J. Biol. Chem., 282, 28106-28116.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain JRG 3456 Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapour diffusion method with 15-20% PEG2000 monomethyl ether, 10% propanol, 0.2% NaN3 in 60 mM HEPES buffer (pH 7.05) Escherichia coli

Protein Variants

Protein Variants Comment Organism
E401A 9.56% of overall wild type activity and 23.4% of wild type activity with 2,6-dichloroindolphenol Escherichia coli
E401K 1.04% of overall wild type activity and 4.63% of wild type activity with 2,6-dichloroindolphenol Escherichia coli
H407A 0.15% of overall wild type activity and 12.0% of wild type activity with 2,6-dichloroindolphenol Escherichia coli
K403A 11.6% of overall wild type activity and 98.0% of wild type activity with 2,6-dichloroindolphenol Escherichia coli
K403E 0.56% of overall wild type activity and 5.46% of wild type activity with 2,6-dichloroindolphenol Escherichia coli
K410A 23.0% of overall wild type activity and 31.6% of wild type activity with 2,6-dichloroindolphenol Escherichia coli
K410E 3.7% of overall wild type activity and 26.1% of wild type activity with 2,6-dichloroindolphenol Escherichia coli
K411A 68.2% of overall wild type activity and 77.5% of wild type activity with 2,6-dichloroindolphenol Escherichia coli
K411E 38.0% of overall wild type activity and 88.0% of wild type activity with 2,6-dichloroindolphenol Escherichia coli
N404A 1.81% of overall wild type activity and 45.1% of wild type activity with 2,6-dichloroindolphenol Escherichia coli
Q408A 31.5% of overall wild type activity and 112.1% of wild type activity with 2,6-dichloroindolphenol Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1304
-
pyruvate mutant enzyme Q408A, with 2,6-dichlorophenolindophenol, at 30°C Escherichia coli
0.1714
-
pyruvate mutant enzyme K410A, with 2,6-dichlorophenolindophenol, at 30°C Escherichia coli
0.2012
-
pyruvate mutant enzyme K411E, with 2,6-dichlorophenolindophenol, at 30°C Escherichia coli
0.2336
-
pyruvate mutant enzyme K411A, with 2,6-dichlorophenolindophenol, at 30°C Escherichia coli
0.2609
-
pyruvate wild type enzyme, with 2,6-dichlorophenolindophenol, at 30°C Escherichia coli
0.6286
-
pyruvate mutant enzyme E401A, with 2,6-dichlorophenolindophenol, at 30°C Escherichia coli
0.7008
-
pyruvate mutant enzyme K403A, with 2,6-dichlorophenolindophenol, at 30°C Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AFG8
-
-

Purification (Commentary)

Purification (Comment) Organism
TSK DEAE-5PW HPLC column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + CoA + NAD+
-
Escherichia coli acetyl-CoA + CO2 + NADH
-
?
pyruvate + CoA + oxidized 2,6-dichlorophenolindophenol
-
Escherichia coli acetyl-CoA + CO2 + reduced 2,6-dichlorophenolindophenol
-
?

Synonyms

Synonyms Comment Organism
E1ec
-
Escherichia coli
pyruvate dehydrogenase complex E1 component
-
Escherichia coli
pyruvate:NAD oxidoreductase
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.081
-
pyruvate mutant enzyme H407A, with 2,6-dichlorophenolindophenol, at 30°C Escherichia coli
0.21
-
pyruvate mutant enzyme K403E, with 2,6-dichlorophenolindophenol, at 30°C Escherichia coli
0.39
-
pyruvate mutant enzyme E401K, with 2,6-dichlorophenolindophenol, at 30°C Escherichia coli
1.53
-
pyruvate mutant enzyme K410E, with 2,6-dichlorophenolindophenol, at 30°C Escherichia coli
3.07
-
pyruvate mutant enzyme N404A, with 2,6-dichlorophenolindophenol, at 30°C Escherichia coli
3.64
-
pyruvate mutant enzyme E401A, with 2,6-dichlorophenolindophenol, at 30°C Escherichia coli
4.41
-
pyruvate mutant enzyme K403A, with 2,6-dichlorophenolindophenol, at 30°C Escherichia coli
7.68
-
pyruvate mutant enzyme K410A, with 2,6-dichlorophenolindophenol, at 30°C Escherichia coli
19.03
-
pyruvate mutant enzyme Q408A, with 2,6-dichlorophenolindophenol, at 30°C Escherichia coli
25.12
-
pyruvate mutant enzyme K411E, with 2,6-dichlorophenolindophenol, at 30°C Escherichia coli
35.13
-
pyruvate mutant enzyme K411A, with 2,6-dichlorophenolindophenol, at 30°C Escherichia coli
37.9
-
pyruvate wild type enzyme, with 2,6-dichlorophenolindophenol, at 30°C Escherichia coli

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate dependent Escherichia coli