Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli M15 cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
vapour diffusion method with 14-18% PEG 3350, 0.1 mM sodium azide, and 200 mM NaSCN in 50 mM potassium phosphate (pH 8.0) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
S264E | pseudophosphorylation mutant, the preceding binding of substrate to the enzyme's active site via the substrate channel and the subsequent reductive acetylation of the E2 component are severely slowed in the mutant enzyme | Homo sapiens |
S264Q | selectively deficient in pyruvate binding and reductive acetylation of component E2 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P08559 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphorylation | phosphorylation of Ser264 slows the preceding binding of substrate to the enzyme's active site via the substrate channel and the subsequent reductive acetylation of the E2 component | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + CoA + NAD+ | - |
Homo sapiens | acetyl-CoA + CO2 + NADH | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
PDHc-E1 | - |
Homo sapiens |
thiamin-dependent pyruvate dehydrogenase | part of the pyruvate dehydrogenase multienzyme complex PDHc as component E1 | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens | |
thiamine diphosphate | dependent | Homo sapiens |