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Literature summary for 1.2.3.4 extracted from
- Entrapment of sorghum root oxalate oxidase into polyvinyl alcohol membrane (2002), Indian J. Biochem. Biophys., 39, 397-400.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.38 | - |
oxalate | 37°C, pH 5.0, free enzyme | Sorghum bicolor |  |
| 6.23 | - |
oxalate | 30°C, pH 6.5, polyvinyl alcohol membrane immobilized oxalate oxidase | Sorghum bicolor | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oxalate + O2 + 2 H+ | Sorghum bicolor | - |
2 CO2 + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sorghum bicolor | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, Sephadex G-200, DEAE-Sephacel | Sorghum bicolor |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Sorghum bicolor | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| room temperature, immobilized oxalate oxidase between folds of filter paper, 30 d, 50% loss of activity | Sorghum bicolor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oxalate + O2 + 2 H+ | - |
Sorghum bicolor | 2 CO2 + H2O2 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
polyvinyl alcohol membrane immobilized oxalate oxidase | Sorghum bicolor |
| 37 | - |
free enzyme | Sorghum bicolor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
free enzyme | Sorghum bicolor |
| 6.5 | - |
polyvinyl alcohol membrane immobilized oxalate oxidase | Sorghum bicolor |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7 | approx. 50% of maximal activity at pH 5.0 and pH 7.0, respectively, polyvinyl alcohol membrane immobilized oxalate oxidase | Sorghum bicolor |
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Release 2023.1 (January 2023)
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