Application | Comment | Organism |
---|---|---|
medicine | colorimetric or UV-determination of oxalic acid in biological fluids and beverages | Hordeum vulgare |
General Stability | Organism |
---|---|
immobilized on zirkonia coated alkylamine glass, retains 97% of enzyme activity | Hordeum vulgare |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cl- | 1 M concentration 75% inhibition | Musa acuminata | |
Fe3+ | 10 mM concentration 35% inhibition | Musa acuminata | |
NaCl | at 1 mM concentration 43% activity retained by free protein, 85% activity retained by immobilized protein | Hordeum vulgare | |
Pb2+ | 2 mM concentration 57% inhibition, 20 mM concentration 89% inhibition | Musa acuminata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.37 | - |
oxalate | immobilized protein | Hordeum vulgare | |
0.42 | - |
oxalate | - |
Hordeum vulgare | |
1.88 | - |
oxalate | - |
Musa acuminata |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Hordeum vulgare | 5829 | - |
cytosol | - |
Musa acuminata | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
- |
- |
Musa acuminata | - |
banana | - |
Storage Stability | Organism |
---|---|
4°C, under 3 months no loss of activity by free protein, over 2 years immobilized protein | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxalic acid + O2 + 2 H+ | - |
Hordeum vulgare | 2 CO2 + H2O2 | - |
ir | |
oxalic acid + O2 + 2 H+ | - |
Musa acuminata | 2 CO2 + H2O2 | - |
ir |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Hordeum vulgare |
60 | - |
70% activity retained | Musa acuminata |
75 | - |
80% wild type and 20% immobilized protein | Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.2 | - |
- |
Hordeum vulgare |
4 | - |
immobilized protein | Hordeum vulgare |