Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.3.1 extracted from

  • Choo, Y.M.; Pelletier, J.; Atungulu, E.; Leal, W.S.
    Identification and characterization of an antennae-specific aldehyde oxidase from the navel orangeworm (2013), PLoS ONE, 8, e67794.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of recombinant AtraAOX2 with His6-tag at the C-terminal end in a baculovirus expression system Amyelois transitella

Inhibitors

Inhibitors Comment Organism Structure
2,4-dinitrophenol 50 microM, 41.7% residual AtraAOX2 activity Amyelois transitella
4-hydroxymercuribenzoate 50 microM, 55.3% residual AtraAOX2 activity Amyelois transitella
antimycin A 10 microM, 45.6% residual AtraAOX2 activity Amyelois transitella
beta-ecdysone 50 microM, 30.1% residual AtraAOX2 activity Amyelois transitella
beta-estradiol 50 microM, 60.2% residual AtraAOX2 activity Amyelois transitella
Digitonin 125 microM, 69.9% residual AtraAOX2 activity Amyelois transitella
ethanol 2% (v/v), 62.1% residual AtraAOX2 activity Amyelois transitella
Isopropanol 2% (v/v), 67.9% residual AtraAOX2 activity Amyelois transitella
menadione 100 microM, 63.1% residual AtraAOX2 activity Amyelois transitella
methanol 2% (v/v), 33.9% residual AtraAOX2 activity Amyelois transitella
additional information hydroquinone and sodium deoxycholate have no inhibitory effect Amyelois transitella
potassium cyanide 1 mM, 71.8% residual AtraAOX2 activity Amyelois transitella
Quinacrine 1 mM, 34.9% residual AtraAOX2 activity Amyelois transitella
rotenone 10 microM, 89.3% residual AtraAOX2 activity Amyelois transitella
Sodium azide 5 mM, 52.4% residual AtraAOX2 activity Amyelois transitella
Triton X-100 0.01% (v/v), 71.8% residual AtraAOX2 activity Amyelois transitella

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
SDS-PAGE, AtraAOX2 monomer still shows strong activity Amyelois transitella
280000
-
Western blot analysis Amyelois transitella

Organism

Organism UniProt Comment Textmining
Amyelois transitella S5FPI8 navel orangeworm
-

Purification (Commentary)

Purification (Comment) Organism
by Ni-NTA column chromatography Amyelois transitella

Source Tissue

Source Tissue Comment Organism Textmining
antenna male and female Amyelois transitella
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E)-hex-2-enal + H2O + O2
-
Amyelois transitella (2E)-hex-2-enoic acid + H2O2
-
?
(2E)-non-2-enal + H2O + O2
-
Amyelois transitella (2E)-non-2-enoic acid + H2O2
-
?
acetaldehyde + H2O + O2
-
Amyelois transitella acetic acid + H2O2
-
?
acrolein + H2O + O2 results indicate that AtraAOX2 also functions as a xenobiotic-degrading enzyme Amyelois transitella ?
-
?
benzaldehyde + H2O + O2
-
Amyelois transitella benzoic acid + H2O2
-
?
citral + H2O + O2 low AtraAOX2 activity Amyelois transitella (2E)-3,7-dimethylocta-2,6-dienoic acid + H2O2
-
?
heptanal + H2O + O2
-
Amyelois transitella heptanoic acid + H2O2
-
?
hexanal + H2O + O2
-
Amyelois transitella hexanoic acid + H2O2
-
?
additional information AtraAOX2 shows strong activity on aldehyde substrates derived from plants, but weak activity on Z11Z13–16Ald, AtraAOX2 does not degrade the carbamate insecticide aldicarb Amyelois transitella ?
-
?
nonanal + H2O + O2
-
Amyelois transitella nonanoic acid + H2O2
-
?
propanal + H2O + O2 highest AtraAOX2 activity Amyelois transitella propanoic acid + H2O2
-
?
vanillin + H2O + O2 low AtraAOX2 activity Amyelois transitella vanillic acid + H2O2
-
?
xanthine + H2O + O2 lowest AtraAOX2 activity Amyelois transitella ? + H2O2
-
?
Z11Z13-16Ald + H2O + O2 low AtraAOX2 activity Amyelois transitella ? + H2O2
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 140000, SDS-PAGE Amyelois transitella

Synonyms

Synonyms Comment Organism
antennae-specific aldehyde oxidase
-
Amyelois transitella
AtraAOX2
-
Amyelois transitella

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 60 relatively stable from 20 to 60°C but activity drops dramatically at 70°C, AtraAOX2 preheated at 60°C, shows biphasic inactivation, with a rapid decrease from 0 to 10 min, and monotonous decrease thereafter, with an estimated half-life of 33 min Amyelois transitella

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
enzyme activity tested with three different buffers at pH 8, but no significant difference observed Amyelois transitella

pH Range

pH Minimum pH Maximum Comment Organism
4 8
-
Amyelois transitella

General Information

General Information Comment Organism
physiological function AtraAOX2 could be involved in detoxifying plant-derived toxic aldehydes and aldehyde-containing pesticides Amyelois transitella