Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli M15 cells | Oryctolagus cuniculus |
expressed in Escherichia coli M15 cells | Macaca fascicularis |
Protein Variants | Comment | Organism |
---|---|---|
A1023Y | the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme | Macaca fascicularis |
A1081V | the mutant completely loses the high cinchonidine oxidation activity of the wild type enzyme | Oryctolagus cuniculus |
A1083T | the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme | Macaca fascicularis |
A1083T/V1085A | the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme | Macaca fascicularis |
I1032V | the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme | Macaca fascicularis |
K1004Q | the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme | Macaca fascicularis |
K1004Q/K1005R | the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme | Macaca fascicularis |
K1004Q/K1005R/M1009I/V1010I | the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme | Macaca fascicularis |
K1004Q/K1005R/M1009I/V1010I/R1021V/A1023Y | the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme | Macaca fascicularis |
K1004Q/K1005R/M1009I/V1010I/R1021V/A1023Y/I1032V | the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme | Macaca fascicularis |
K1004Q/K1005R/M1009I/V1010I/R1021V/A1023Y/I1032V/G1064K/I1067M | the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme | Macaca fascicularis |
M1009I | the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme | Macaca fascicularis |
V1085A | the mutant acquires high cinchonidine oxidation activity | Macaca fascicularis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0074 | - |
cinchonidine | wild type enzyme, in 80 mM phosphate buffer, pH 7.4, at 37°C | Oryctolagus cuniculus | |
0.0109 | - |
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine | wild type enzyme, in 80 mM phosphate buffer, pH 7.4, at 37°C | Macaca fascicularis | |
0.012 | - |
cinchonidine | mutant enzyme V1085A, in 80 mM phosphate buffer, pH 7.4, at 37°C | Macaca fascicularis | |
0.0157 | - |
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine | mutant enzyme K1004Q/K1005R/M1009I/V1010I/R1021V/A1023Y/I1032V/G1064K/I1067M, in 80 mM phosphate buffer, pH 7.4, at 37°C | Macaca fascicularis | |
0.0244 | - |
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine | mutant enzyme K1004Q/K1005R/M1009I/V1010I/R1021V/A1023Y/I1032V, in 80 mM phosphate buffer, pH 7.4, at 37°C | Macaca fascicularis | |
0.0264 | - |
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine | mutant enzyme A1023Y, in 80 mM phosphate buffer, pH 7.4, at 37°C | Macaca fascicularis | |
0.027 | - |
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine | mutant enzyme M1009I, in 80 mM phosphate buffer, pH 7.4, at 37°C | Macaca fascicularis | |
0.0334 | - |
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine | mutant enzyme I1032V, in 80 mM phosphate buffer, pH 7.4, at 37°C | Macaca fascicularis | |
0.0352 | - |
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine | mutant enzyme A1081V, in 80 mM phosphate buffer, pH 7.4, at 37°C | Oryctolagus cuniculus | |
0.036 | - |
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine | mutant enzyme K1004Q/K1005R, in 80 mM phosphate buffer, pH 7.4, at 37°C | Macaca fascicularis | |
0.0366 | - |
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine | mutant enzyme K1004Q, in 80 mM phosphate buffer, pH 7.4, at 37°C | Macaca fascicularis | |
0.0521 | - |
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine | mutant enzyme K1004Q/K1005R/M1009I/V1010I, in 80 mM phosphate buffer, pH 7.4, at 37°C | Macaca fascicularis | |
0.0965 | - |
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine | mutant enzyme K1004Q/K1005R/M1009I/V1010I/R1021V/A1023Y, in 80 mM phosphate buffer, pH 7.4, at 37°C | Macaca fascicularis | |
0.128 | - |
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine | mutant enzyme A1083T/V1085A, in 80 mM phosphate buffer, pH 7.4, at 37°C | Macaca fascicularis | |
0.131 | - |
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine | mutant enzyme V1085A, in 80 mM phosphate buffer, pH 7.4, at 37°C | Macaca fascicularis | |
0.147 | - |
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine | wild type enzyme, in 80 mM phosphate buffer, pH 7.4, at 37°C | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Macaca fascicularis | - |
- |
- |
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni affinity column chromatography | Oryctolagus cuniculus |
Ni affinity column chromatography | Macaca fascicularis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine + H2O + O2 | i.e. (S)-RS-8359 | Macaca fascicularis | ? | - |
? | |
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine + H2O + O2 | i.e. (S)-RS-8359 | Oryctolagus cuniculus | ? | - |
? | |
cinchonidine + H2O + O2 | rabbit AOX1 has an extremely high Vmax value toward cinchonidine | Oryctolagus cuniculus | ? | - |
? | |
cinchonidine + H2O + O2 | wild type monkey AOX1 is not able to oxidize chinchonidine, but mutant enzyme V1085A does | Macaca fascicularis | ? | - |
? | |
zonisamide + H2O + O2 | - |
Oryctolagus cuniculus | ? | - |
? | |
zonisamide + H2O + O2 | - |
Macaca fascicularis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aldehyde oxidase 1 | - |
Oryctolagus cuniculus |
aldehyde oxidase 1 | - |
Macaca fascicularis |
AOX1 | - |
Oryctolagus cuniculus |
AOX1 | - |
Macaca fascicularis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Oryctolagus cuniculus | |
FAD | - |
Macaca fascicularis |