Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.3.1 extracted from

  • Maeda, Y.; Doubayashi, D.; Ootake, T.; Oki, M.; Mikami, B.; Uchida, H.
    Crystallization and preliminary X-ray analysis of formate oxidase, an enzyme of the glucose-methanol-choline oxidoreductase family (2010), Acta Crystallogr. Sect. F, 66, 1064-1066.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Aspergillus oryzae

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1 M sodium acetate buffer pH 4.6 and 6% (w/v) PEG 4000, at 20°C Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-
Aspergillus oryzae RIB 40
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni Sepharose 6 Fast Flow column chromatography, HiTrap QHP column chromatography, and HisTrap FF column chromatography Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + O2
-
Aspergillus oryzae CO2 + H2O2
-
?
formate + O2
-
Aspergillus oryzae RIB 40 CO2 + H2O2
-
?

Subunits

Subunits Comment Organism
trimer x-ray crystallography Aspergillus oryzae

Synonyms

Synonyms Comment Organism
FOD
-
Aspergillus oryzae
formate oxidase
-
Aspergillus oryzae

Cofactor

Cofactor Comment Organism Structure
FAD
-
Aspergillus oryzae