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Literature summary for 1.2.1.46 extracted from

  • Ben Ali, M.; Gonchar, M.; Gayda, G.; Paryzhak, S.; Maaref, M.A.; Jaffrezic-Renault, N.; Korpan, Y.
    Formaldehyde-sensitive sensor based on recombinant formaldehyde dehydrogenase using capacitance versus voltage measurements (2007), Biosens. Bioelectron., 22, 2790-2795.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information best sensitivity of the biosensor is observed in 2.5 mM borate buffer, pH 8.4 Ogataea angusta

Application

Application Comment Organism
industry construction of a formaldehyde-selective biosensor using NAD+- and glutathione-dependent recombinant formaldehyde dehydrogenase as a bio-recognition element immobilised on the surface of Si/SiO2/Si3N4 structure Ogataea angusta

Cloned(Commentary)

Cloned (Comment) Organism
FLD1 gene with the own promoter inserted into the integrative plasmid pYT1 containing LEU2 gene of Saccharomyces cerevisiae (as a selective marker). The constructed vector is used for multi-copy integration of the target gene into genome by transformation of the recipient cells of the strain NCYC 495 Ogataea angusta

Inhibitors

Inhibitors Comment Organism Structure
additional information biosensor output signal decreases substantially with the increase of borate buffer concentration. Sensitivity of the biosensor in 2.5 mM Tris-HCl buffer is decreased dramatically Ogataea angusta

Organism

Organism UniProt Comment Textmining
Ogataea angusta
-
-
-
Ogataea angusta Tf 11-6
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by ion-exchange chromatography Ogataea angusta

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12
-
at 20°C Ogataea angusta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formaldehyde + NAD+ + H2O
-
Ogataea angusta formate + NADH + H+
-
?
formaldehyde + NAD+ + H2O
-
Ogataea angusta Tf 11-6 formate + NADH + H+
-
?
formaldehyde + NAD+ + H2O
-
Ogataea angusta Tf-11-6 formate + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
FDH
-
Ogataea angusta
NAD+- and glutathione-dependent formaldehyde dehydrogenase
-
Ogataea angusta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
best sensitivity of the biosensor output signal is observed in 2.5 mM borate buffer, pH 8.4 Ogataea angusta

Cofactor

Cofactor Comment Organism Structure
glutathione
-
Ogataea angusta
NAD+
-
Ogataea angusta