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Literature summary for 1.2.1.3 extracted from

  • Li, X.; Li, Y.; Wei, D.; Li, P.; Wang, L.; Feng, L.
    Characterization of a broad-range aldehyde dehydrogenase involved in alkane degradation in Geobacillus thermodenitrificans NG80-2 (2010), Microbiol. Res., 165, 706-712.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 2% (v/v) Triton X-100 enhances the enzyme activity by 270% Geobacillus thermodenitrificans

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Geobacillus thermodenitrificans

Inhibitors

Inhibitors Comment Organism Structure
Co2+ complete inhibition at 1 mM Geobacillus thermodenitrificans
Cu2+ complete inhibition at 1 mM Geobacillus thermodenitrificans
Fe3+ complete inhibition at 1 mM Geobacillus thermodenitrificans
additional information EDTA has no effect on ALDH activity Geobacillus thermodenitrificans
Ni2+ complete inhibition at 1 mM Geobacillus thermodenitrificans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0066
-
acetaldehyde in 50 mM KH2PO4-K2HPO4 buffer (pH 8.0), at 60°C Geobacillus thermodenitrificans
0.389
-
NAD+ in 50 mM KH2PO4-K2HPO4 buffer (pH 8.0), at 60°C Geobacillus thermodenitrificans

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 150% activity at 1 mM Geobacillus thermodenitrificans
K+ 118% activity at 1 mM Geobacillus thermodenitrificans
Mg2+ 180% activity at 1 mM Geobacillus thermodenitrificans
Mn2+ 140% activity at 1 mM Geobacillus thermodenitrificans
additional information 1 mM Na+ has no effect on the enzyme activity Geobacillus thermodenitrificans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56532
-
4 * 56532, calculated from amino acid sequence Geobacillus thermodenitrificans
57000
-
4 * 57000, SDS-PAGE Geobacillus thermodenitrificans
220000
-
major band, gel filtration Geobacillus thermodenitrificans
440000
-
minor band, gel filtration Geobacillus thermodenitrificans

Organism

Organism UniProt Comment Textmining
Geobacillus thermodenitrificans
-
-
-
Geobacillus thermodenitrificans NG80-2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
chelating Sepharose column chromatography, gel filtration Geobacillus thermodenitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chlorobenzaldehyde + NAD+ + H2O 28.35% activity compared to formaldehyde Geobacillus thermodenitrificans 2-chlorobenzoate + NADH + H+
-
?
2-phthalaldehyde + NAD+ + H2O 10.81% activity compared to formaldehyde Geobacillus thermodenitrificans phthalate + NADH + H+
-
?
acetaldehyde + NAD+ + H2O 77.68% activity compared to formaldehyde Geobacillus thermodenitrificans acetate + NADH + H+
-
?
acetaldehyde + NAD+ + H2O 77.68% activity compared to formaldehyde Geobacillus thermodenitrificans NG80-2 acetate + NADH + H+
-
?
benzaldehyde + NAD+ + H2O 72.38% activity compared to formaldehyde Geobacillus thermodenitrificans benzoate + NADH + H+
-
?
benzaldehyde + NAD+ + H2O 72.38% activity compared to formaldehyde Geobacillus thermodenitrificans NG80-2 benzoate + NADH + H+
-
?
butyraldehyde + NAD+ + H2O 71.65% activity compared to formaldehyde Geobacillus thermodenitrificans butyrate + NADH + H+
-
?
butyraldehyde + NAD+ + H2O 71.65% activity compared to formaldehyde Geobacillus thermodenitrificans NG80-2 butyrate + NADH + H+
-
?
decanaldehyde + NAD+ + H2O 50.3% activity compared to formaldehyde Geobacillus thermodenitrificans decanoate + NADH + H+
-
?
dodecanaldehyde + NAD+ + H2O 42.02% activity compared to formaldehyde Geobacillus thermodenitrificans dodecanoate + NADH + H+
-
?
eicosanoic aldehyde + NAD+ + H2O 8.79% activity compared to formaldehyde Geobacillus thermodenitrificans eicosanoate + NADH + H+
-
?
formaldehyde + NAD+ + H2O 100% activity Geobacillus thermodenitrificans formate + NADH + H+
-
?
hexadecanal + NAD+ + H2O 15.77% activity compared to formaldehyde Geobacillus thermodenitrificans hexadecanoate + NADH + H+
-
?
hexaldehyde + NAD+ + H2O 76.21% activity compared to formaldehyde Geobacillus thermodenitrificans hexanoate + NADH + H+
-
?
additional information no activity towards NADP+, tetracosaldehyde, 2-hydroxylbenzaldehyde, ethanol, hexadecanol, acetate, and hexadecanoic acid Geobacillus thermodenitrificans ?
-
?
additional information the ALDH has no reductive activity on acetate or hexadecanoic acid Geobacillus thermodenitrificans ?
-
?
additional information no activity towards NADP+, tetracosaldehyde, 2-hydroxylbenzaldehyde, ethanol, hexadecanol, acetate, and hexadecanoic acid Geobacillus thermodenitrificans NG80-2 ?
-
?
additional information the ALDH has no reductive activity on acetate or hexadecanoic acid Geobacillus thermodenitrificans NG80-2 ?
-
?
octadecanal + NAD+ + H2O 12% activity compared to formaldehyde Geobacillus thermodenitrificans octadecanoate + NADH + H+
-
?
octyl aldehyde + NAD+ + H2O 72.27% activity compared to formaldehyde Geobacillus thermodenitrificans octanoate + NADH + H+
-
?
phenylacetaldehyde + NAD+ + H2O 37.86% activity compared to formaldehyde Geobacillus thermodenitrificans phenylacetate + NADH + H+
-
?
tetradecyl aldehyde + NAD+ + H2O 11.71% activity compared to formaldehyde Geobacillus thermodenitrificans tetradecanoate + NADH + H+
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 57000, SDS-PAGE Geobacillus thermodenitrificans
homotetramer 4 * 56532, calculated from amino acid sequence Geobacillus thermodenitrificans

Synonyms

Synonyms Comment Organism
ALDH
-
Geobacillus thermodenitrificans
irreversible NAD+-dependent aldehyde dehydrogenase
-
Geobacillus thermodenitrificans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Geobacillus thermodenitrificans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 70
-
Geobacillus thermodenitrificans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 80 the purified enzyme retains 97%, 85%, 71%, and 56% of the original activity, respectively, after incubation at 40, 50, 60 and 70°C for 60 min. The enzyme is inactivated after incubation at 80°C Geobacillus thermodenitrificans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Geobacillus thermodenitrificans

pH Range

pH Minimum pH Maximum Comment Organism
6 8.8
-
Geobacillus thermodenitrificans

Cofactor

Cofactor Comment Organism Structure
NAD+ dependent on Geobacillus thermodenitrificans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.006
-
NAD+ in 50 mM KH2PO4-K2HPO4 buffer (pH 8.0), at 60°C Geobacillus thermodenitrificans
0.49
-
acetaldehyde in 50 mM KH2PO4-K2HPO4 buffer (pH 8.0), at 60°C Geobacillus thermodenitrificans