Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | 2% (v/v) Triton X-100 enhances the enzyme activity by 270% | Geobacillus thermodenitrificans |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Geobacillus thermodenitrificans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | complete inhibition at 1 mM | Geobacillus thermodenitrificans | |
Cu2+ | complete inhibition at 1 mM | Geobacillus thermodenitrificans | |
Fe3+ | complete inhibition at 1 mM | Geobacillus thermodenitrificans | |
additional information | EDTA has no effect on ALDH activity | Geobacillus thermodenitrificans | |
Ni2+ | complete inhibition at 1 mM | Geobacillus thermodenitrificans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0066 | - |
acetaldehyde | in 50 mM KH2PO4-K2HPO4 buffer (pH 8.0), at 60°C | Geobacillus thermodenitrificans | |
0.389 | - |
NAD+ | in 50 mM KH2PO4-K2HPO4 buffer (pH 8.0), at 60°C | Geobacillus thermodenitrificans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 150% activity at 1 mM | Geobacillus thermodenitrificans | |
K+ | 118% activity at 1 mM | Geobacillus thermodenitrificans | |
Mg2+ | 180% activity at 1 mM | Geobacillus thermodenitrificans | |
Mn2+ | 140% activity at 1 mM | Geobacillus thermodenitrificans | |
additional information | 1 mM Na+ has no effect on the enzyme activity | Geobacillus thermodenitrificans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56532 | - |
4 * 56532, calculated from amino acid sequence | Geobacillus thermodenitrificans |
57000 | - |
4 * 57000, SDS-PAGE | Geobacillus thermodenitrificans |
220000 | - |
major band, gel filtration | Geobacillus thermodenitrificans |
440000 | - |
minor band, gel filtration | Geobacillus thermodenitrificans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus thermodenitrificans | - |
- |
- |
Geobacillus thermodenitrificans NG80-2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
chelating Sepharose column chromatography, gel filtration | Geobacillus thermodenitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-chlorobenzaldehyde + NAD+ + H2O | 28.35% activity compared to formaldehyde | Geobacillus thermodenitrificans | 2-chlorobenzoate + NADH + H+ | - |
? | |
2-phthalaldehyde + NAD+ + H2O | 10.81% activity compared to formaldehyde | Geobacillus thermodenitrificans | phthalate + NADH + H+ | - |
? | |
acetaldehyde + NAD+ + H2O | 77.68% activity compared to formaldehyde | Geobacillus thermodenitrificans | acetate + NADH + H+ | - |
? | |
acetaldehyde + NAD+ + H2O | 77.68% activity compared to formaldehyde | Geobacillus thermodenitrificans NG80-2 | acetate + NADH + H+ | - |
? | |
benzaldehyde + NAD+ + H2O | 72.38% activity compared to formaldehyde | Geobacillus thermodenitrificans | benzoate + NADH + H+ | - |
? | |
benzaldehyde + NAD+ + H2O | 72.38% activity compared to formaldehyde | Geobacillus thermodenitrificans NG80-2 | benzoate + NADH + H+ | - |
? | |
butyraldehyde + NAD+ + H2O | 71.65% activity compared to formaldehyde | Geobacillus thermodenitrificans | butyrate + NADH + H+ | - |
? | |
butyraldehyde + NAD+ + H2O | 71.65% activity compared to formaldehyde | Geobacillus thermodenitrificans NG80-2 | butyrate + NADH + H+ | - |
? | |
decanaldehyde + NAD+ + H2O | 50.3% activity compared to formaldehyde | Geobacillus thermodenitrificans | decanoate + NADH + H+ | - |
? | |
dodecanaldehyde + NAD+ + H2O | 42.02% activity compared to formaldehyde | Geobacillus thermodenitrificans | dodecanoate + NADH + H+ | - |
? | |
eicosanoic aldehyde + NAD+ + H2O | 8.79% activity compared to formaldehyde | Geobacillus thermodenitrificans | eicosanoate + NADH + H+ | - |
? | |
formaldehyde + NAD+ + H2O | 100% activity | Geobacillus thermodenitrificans | formate + NADH + H+ | - |
? | |
hexadecanal + NAD+ + H2O | 15.77% activity compared to formaldehyde | Geobacillus thermodenitrificans | hexadecanoate + NADH + H+ | - |
? | |
hexaldehyde + NAD+ + H2O | 76.21% activity compared to formaldehyde | Geobacillus thermodenitrificans | hexanoate + NADH + H+ | - |
? | |
additional information | no activity towards NADP+, tetracosaldehyde, 2-hydroxylbenzaldehyde, ethanol, hexadecanol, acetate, and hexadecanoic acid | Geobacillus thermodenitrificans | ? | - |
? | |
additional information | the ALDH has no reductive activity on acetate or hexadecanoic acid | Geobacillus thermodenitrificans | ? | - |
? | |
additional information | no activity towards NADP+, tetracosaldehyde, 2-hydroxylbenzaldehyde, ethanol, hexadecanol, acetate, and hexadecanoic acid | Geobacillus thermodenitrificans NG80-2 | ? | - |
? | |
additional information | the ALDH has no reductive activity on acetate or hexadecanoic acid | Geobacillus thermodenitrificans NG80-2 | ? | - |
? | |
octadecanal + NAD+ + H2O | 12% activity compared to formaldehyde | Geobacillus thermodenitrificans | octadecanoate + NADH + H+ | - |
? | |
octyl aldehyde + NAD+ + H2O | 72.27% activity compared to formaldehyde | Geobacillus thermodenitrificans | octanoate + NADH + H+ | - |
? | |
phenylacetaldehyde + NAD+ + H2O | 37.86% activity compared to formaldehyde | Geobacillus thermodenitrificans | phenylacetate + NADH + H+ | - |
? | |
tetradecyl aldehyde + NAD+ + H2O | 11.71% activity compared to formaldehyde | Geobacillus thermodenitrificans | tetradecanoate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 57000, SDS-PAGE | Geobacillus thermodenitrificans |
homotetramer | 4 * 56532, calculated from amino acid sequence | Geobacillus thermodenitrificans |
Synonyms | Comment | Organism |
---|---|---|
ALDH | - |
Geobacillus thermodenitrificans |
irreversible NAD+-dependent aldehyde dehydrogenase | - |
Geobacillus thermodenitrificans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Geobacillus thermodenitrificans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | - |
Geobacillus thermodenitrificans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 80 | the purified enzyme retains 97%, 85%, 71%, and 56% of the original activity, respectively, after incubation at 40, 50, 60 and 70°C for 60 min. The enzyme is inactivated after incubation at 80°C | Geobacillus thermodenitrificans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Geobacillus thermodenitrificans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.8 | - |
Geobacillus thermodenitrificans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent on | Geobacillus thermodenitrificans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
NAD+ | in 50 mM KH2PO4-K2HPO4 buffer (pH 8.0), at 60°C | Geobacillus thermodenitrificans | |
0.49 | - |
acetaldehyde | in 50 mM KH2PO4-K2HPO4 buffer (pH 8.0), at 60°C | Geobacillus thermodenitrificans |