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Literature summary for 1.2.1.3 extracted from
- Gene cloning and characterization of an aldehyde dehydrogenase from long-chain alkane-degrading Geobacillus thermoleovorans B23 (2009), Extremophiles, 14, 33-39.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Geobacillus thermoleovorans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Geobacillus thermoleovorans |  |
| Zn2+ | - |
Geobacillus thermoleovorans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | the activity is elevated to 2-3fold in the presence of 2 mM Ba2+ | Geobacillus thermoleovorans | |
| Ca2+ | the activity is elevated to 2-3fold in the presence of 2 mM Ca2+ | Geobacillus thermoleovorans | |
| Sr2+ | the activity is elevated to 2-3fold in the presence of 2 mM Sr2+ | Geobacillus thermoleovorans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53886 | - |
8 * 53886, calculated from amino acid sequence | Geobacillus thermoleovorans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus thermoleovorans | - |
- |
- |
| Geobacillus thermoleovorans B23 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, column chromatography, gel filtration | Geobacillus thermoleovorans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tetradecanal + NAD+ + H2O | high activity | Geobacillus thermoleovorans | tetradecanoate + NADH | - |
? | |
| tetradecanal + NAD+ + H2O | high activity | Geobacillus thermoleovorans B23 | tetradecanoate + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homooctamer | 8 * 53886, calculated from amino acid sequence | Geobacillus thermoleovorans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Bt-Aldh | - |
Geobacillus thermoleovorans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 55 | - |
Geobacillus thermoleovorans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Geobacillus thermoleovorans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Geobacillus thermoleovorans | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Geobacillus thermoleovorans | transcription level of Bt-aldh is increased upon alkane degradation | up |
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Release 2023.1 (January 2023)
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