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Literature summary for 1.2.1.3 extracted from

  • Jo, J.E.; Mohan Raj, S.; Rathnasingh, C.; Selvakumar, E.; Jung, W.C.; Park, S.
    Cloning, expression, and characterization of an aldehyde dehydrogenase from Escherichia coli K-12 that utilizes 3-hydroxypropionaldehyde as a substrate (2008), Appl. Microbiol. Biotechnol., 81, 51-60.
    View publication on PubMed
Search for more literature for 1.2.1.3

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 27% increased activity at 1 mM Escherichia coli
dithiothreitol 75% increased activity at 1 mM Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Ag+ complete inhibition at 1 mM Escherichia coli
Co2+ 50.3% residual activity at 1 mM Escherichia coli
Cu2+ 2.9% residual activity at 1 mM Escherichia coli
EDTA 85.3% residual activity at 1 mM Escherichia coli
Fe2+ 57.5% residual activity at 1 mM Escherichia coli
Hg2+ complete inhibition at 1 mM Escherichia coli
K+ 95.3% residual activity at 1 mM Escherichia coli
Li+ 2.8% residual activity at 1 mM Escherichia coli
Mg2+ 80.6% residual activity at 1 mM Escherichia coli
Mn2+ 84.8% residual activity at 1 mM Escherichia coli
Na+ 83.6% residual activity at 1 mM Escherichia coli
NH3 79.8% residual activity at 1 mM Escherichia coli
Ni2+ 72.4% residual activity at 1 mM Escherichia coli
Semicarbazide hydrochloride 86.7% residual activity at 1 mM Escherichia coli
Sodium bisulfite 1.8% residual activity at 1 mM Escherichia coli
Zn2+ 7% residual activity at 1 mM Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 3-hydroxypropionic acid with NADH as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C Escherichia coli
0.24
-
 Valeraldehyde with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C Escherichia coli
0.29
-
 3-hydroxypropionaldehyde with NADP+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C Escherichia coli
0.49
-
 3-hydroxypropionaldehyde with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C Escherichia coli
0.68
-
 Isovaleraldehyde with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C Escherichia coli
0.97
-
 Butyraldehyde with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C Escherichia coli
1
-
 acetaldehyde with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C Escherichia coli
1.21
-
 propionaldehyde with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C Escherichia coli
5.37
-
 benzaldehyde with NADP+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 recombinant enzyme, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA-HP resin column chromatography Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.42
-
 crude extract, using 3-hydroxypropionaldehyde as a substrate, with NAD+ as a cofactor, at pH 8.0 and 37°C Escherichia coli
38.1
-
 after 26.8fold purification, using 3-hydroxypropionaldehyde as a substrate, with NAD+ as a cofactor, at pH 8.0 and 37°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-furaldehyde + NAD+ + H2O
-
 Escherichia coli 2-furanoic acid + NADH + H+
-
 ?
3-hydroxypropanoate + NADH + 2 H+
-
 Escherichia coli 3-hydroxypropionaldehyde + NAD+ + H2O
-
 r
3-hydroxypropionaldehyde + NAD+ + H2O
-
 Escherichia coli 3-hydroxypropanoate + NADH + 2 H+
-
 r
acetaldehyde + NAD+ + H2O
-
 Escherichia coli acetate + NADH + H+
-
 ?
benzaldehyde + NADP+ + H2O
-
 Escherichia coli benzoate + NADPH + H+
-
 ?
butyraldehyde + NAD+ + H2O
-
 Escherichia coli butanoic acid + NADH + H+
-
 ?
isovaleraldehyde + NAD+ + H2O
-
 Escherichia coli isovalerate + NADH + H+
-
 ?
additional information no activity towards 3-hydroxypropionic acid in the presence of NADPH and formaldehyde Escherichia coli ?
-
 ?
propionaldehyde + NAD+ + H2O
-
 Escherichia coli propanoic acid + NADH + H+
-
 ?
valeraldehyde + NAD+ + H2O
-
 Escherichia coli valerate + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
ALDH
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 at pH 7 Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 50 AldH appears to be stable between 20°C and 50 C but becomes very unstable at or above 50°C Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.26
-
 3-hydroxypropionic acid with NADH as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C Escherichia coli
4.91
-
 3-hydroxypropionaldehyde with NADP+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C Escherichia coli
11.03
-
 acetaldehyde with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C Escherichia coli
17.37
-
 benzaldehyde with NADP+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C Escherichia coli
24.35
-
 propionaldehyde with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C Escherichia coli
26.24
-
 Valeraldehyde with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C Escherichia coli
26.95
-
 Butyraldehyde with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C Escherichia coli
27.31
-
 Isovaleraldehyde with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C Escherichia coli
28.54
-
 3-hydroxypropionaldehyde with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
4 10
-
 Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 increasing or decreasing one pH unit from the optimal (pH 8.0) attenuates more than 70% of activity, pH 6.0 only 6% of the activity remains, pH values below 5.0 completely abolish activity Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+ prefers NAD+ over NADP+ Escherichia coli
NADP+ prefers NAD+ over NADP+, in case of 3-hydroxypropionaldehyde, the activity is decreased by 73% when NAD+ is replaced with NADP+ Escherichia coli