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Literature summary for 1.2.1.3 extracted from

  • Bedino, S.; Testore, G.; Obert, F.
    Initial characterization of aldehyde dehydrogenase from rat testis cytosol (1990), Biol. Chem. Hoppe-Seyler, 371, 95-101.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 100 mM, full activation Rattus norvegicus
dithiothreitol 4 mM, full activation Rattus norvegicus
GSH 30% activation Rattus norvegicus

General Stability

General Stability Organism
freezing causes loss of activity Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
benzaldehyde substrate inhibition Rattus norvegicus
Butanal substrate inhibition Rattus norvegicus
Ca2+ 0.4 mM, 50% inhibition Rattus norvegicus
D-glyceraldehyde substrate inhibition Rattus norvegicus
hexanal substrate inhibition Rattus norvegicus
Isobutanal substrate inhibition Rattus norvegicus
Mg2+ 0.4 mM, 50% inhibition Rattus norvegicus
pentanal substrate inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
hexanal
-
Rattus norvegicus
0.006
-
benzaldehyde
-
Rattus norvegicus
0.006
-
pentanal
-
Rattus norvegicus
0.007
-
Butanal
-
Rattus norvegicus
0.023
-
propanal
-
Rattus norvegicus
0.03
-
Isobutanal
-
Rattus norvegicus
0.05
-
NAD+
-
Rattus norvegicus
0.246
-
acetaldehyde
-
Rattus norvegicus
1.136
-
D-glyceraldehyde
-
Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
testis
-
Rattus norvegicus
-

Storage Stability

Storage Stability Organism
4°C, phosphate buffer pH 7.4, containing 4 mM dithiothreitol or 10 mM 2-mercaptoethanol, stable for 8-10 days Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NAD+ + H2O
-
Rattus norvegicus acetate + NADH + H+
-
?
benzaldehyde + NAD+ + H2O
-
Rattus norvegicus benzoate + NADH + H+
-
?
butanal + NAD+ + H2O
-
Rattus norvegicus butyrate + NADH + H+
-
?
D-glyceraldehyde + NAD+ + H2O
-
Rattus norvegicus D-glycerate + NADH + H+
-
r
glyceraldehyde + NAD+ + H2O
-
Rattus norvegicus glycerate + NADH
-
?
hexanal + NAD+ + H2O
-
Rattus norvegicus hexanoate + NADH + H+
-
?
isobutanal + NAD+ + H2O
-
Rattus norvegicus isobutyrate + NADH
-
?
pentanal + NAD+ + H2O
-
Rattus norvegicus pentanoate + NADH
-
?
propanal + NAD+ + H2O
-
Rattus norvegicus propionate + NADH
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
90 min, pH 7.4, 0.2 M phosphate, 4 mM dithiothreitol, stable Rattus norvegicus
55
-
30 min, 20% loss of activity of diluted solution, 70% loss of activity of concentrated preparations Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
7.3 9.5 50% of maximal activity at pH 7.3 and at pH 9.5 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.17
-
hexanal
-
Rattus norvegicus
0.37
-
benzaldehyde
-
Rattus norvegicus
0.54
-
pentanal
-
Rattus norvegicus
4.35
-
Butanal
-
Rattus norvegicus
14.32
-
D-glyceraldehyde
-
Rattus norvegicus
100.1
-
Isobutanal
-
Rattus norvegicus