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Literature summary for 1.2.1.28 extracted from

  • Inoue, J.; Shaw, J.P.; Rekik, M.; Harayama, S.
    Overlapping substrate specificities of benzaldehyde dehydrogenase (the xylC gene product) and 2-hydroxymuconic semialdehyde dehydrogenase (the xylG gene product) encoded by TOL plasmid pWW0 of Pseudomonas putida (1995), J. Bacteriol., 177, 1196-1201.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.28

General Stability

General Stability Organism
dithiothreitol stabilizes Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0015
-
 4-methylbenzaldehyde
-
 Pseudomonas putida
0.0019
-
 3-methylbenzaldehyde
-
 Pseudomonas putida
0.0021
-
 3-Chlorobenzaldehyde
-
 Pseudomonas putida
0.0025
-
 benzaldehyde
-
 Pseudomonas putida
0.0026
-
 3-Methoxybenzaldehyde
-
 Pseudomonas putida
0.0029
-
 3-Fluorobenzaldehyde
-
 Pseudomonas putida
0.022
-
 3-Nitrobenzaldehyde
-
 Pseudomonas putida
0.32
-
 NAD+
-
 Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51941
-
 4 * 51941, deduced from nucleotide sequence Pseudomonas putida
52500
-
 4 * 52500, SDS-PAGE Pseudomonas putida
122000 126000 gel filtration Pseudomonas putida
208000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzaldehyde + NAD+ + H2O Pseudomonas putida
-
 benzoate + NADH + H+
-
 ?
benzaldehyde + NAD+ + H2O Pseudomonas putida KT 2240
-
 benzoate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-chlorobenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 3-chlorobenzoate + NADH
-
 ?
3-chlorobenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida KT 2240 3-chlorobenzoate + NADH
-
 ?
3-fluorobenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 3-fluorobenzoate + NADH + H+
-
 ?
3-methoxybenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 3-methoxybenzoate + NADH
-
 ?
3-methylbenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 3-methylbenzoate + NADH
-
 ?
3-nitrobenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 3-nitrobenzoate + NADH
-
 ?
3-nitrobenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida KT 2240 3-nitrobenzoate + NADH
-
 ?
4-carboxybenzaldehyde + NAD+ + H2O 1650% of activity with benzaldehyde Pseudomonas putida 4-carboxybenzoate + NADH
-
 ?
4-carboxybenzaldehyde + NAD+ + H2O 1650% of activity with benzaldehyde Pseudomonas putida KT 2240 4-carboxybenzoate + NADH
-
 ?
4-methoxybenzaldehyde + NAD+ + H2O 90% of activity with benzaldehyde Pseudomonas putida 4-methoxybenzoate + NADH + H+
-
 ?
4-methylbenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 4-methylbenzoate + NADH
-
 ?
benzaldehyde + NAD+ + H2O
-
 Pseudomonas putida benzoate + NADH + H+
-
 ?
benzaldehyde + NAD+ + H2O
-
 Pseudomonas putida benzoate + NADH + H+
-
 ir
benzaldehyde + NAD+ + H2O
-
 Pseudomonas putida KT 2240 benzoate + NADH + H+
-
 ?
benzaldehyde + NAD+ + H2O
-
 Pseudomonas putida KT 2240 benzoate + NADH + H+
-
 ir
pyridine 4-carboxyaldehyde + NAD+ + H2O 1380% of activity with benzaldehyde Pseudomonas putida pyridine 4-formiate + NADH
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 52500, SDS-PAGE Pseudomonas putida
tetramer 4 * 51941, deduced from nucleotide sequence Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Pseudomonas putida

pH Range

pH Minimum pH Maximum Comment Organism
6 10
-
 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pseudomonas putida