Literature summary for 1.2.1.12 extracted from

Machado, A.; Silva, M.; Iulek, J.
Expression, purification, enzymatic characterization and crystallization of glyceraldehyde-3-phosphate dehydrogenase from Naegleria gruberi, the first one from phylum Percolozoa (2016), Protein Expr. Purif., 127, 125-130 .

Activating Compound

Activating Compound	Comment	Organism	Structure
arsenate	best at 10 mM and up to 30 mM	Naegleria gruberi

Cloned(Commentary)

Cloned (Comment)	Organism
GAPDH gene, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)	Naegleria gruberi

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant N-terminally His-tagged enzyme, hanging drop vapor diffusion method, mixing of 10 mg/ml protein with reservoir solution containing either 12.9% w/v PEG 2000, 50 mM acetate buffer, pH 5.2, and 6.4% w/v PEG 200, or 2.5% w/v PEG 1000, 50 mM acetate buffer, pH 4.8, and 25.7% w/v PEG 2000 MME, 18°C, 3 days, X-ray diffraction structure determination and analysis at 2.40 A and 1.94 A resolutions, respectively	Naegleria gruberi

Crystallization (Comment)

Organism

purified recombinant N-terminally His-tagged enzyme, hanging drop vapor diffusion method, mixing of 10 mg/ml protein with reservoir solution containing either 12.9% w/v PEG 2000, 50 mM acetate buffer, pH 5.2, and 6.4% w/v PEG 200, or 2.5% w/v PEG 1000, 50 mM acetate buffer, pH 4.8, and 25.7% w/v PEG 2000 MME, 18°C, 3 days, X-ray diffraction structure determination and analysis at 2.40 A and 1.94 A resolutions, respectively

Naegleria gruberi

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	the enzyme presents allosteric positive cooperativity for substrates NAD+ and D-glyceraldehyde 3-phosphate, kinetic analysis, overview	Naegleria gruberi

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+	Naegleria gruberi	-	3-phospho-D-glyceroyl phosphate + NADH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Naegleria gruberi	-	nonpathogenic, free-living amoeba	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickell affinity chromatography, dialysis, and gel filtration	Naegleria gruberi

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+	-	Naegleria gruberi	3-phospho-D-glyceroyl phosphate + NADH + H+	-	?

Synonyms

Synonyms	Comment	Organism
GAPDH	-	Naegleria gruberi
glyceraldehyde-3-phosphate dehydrogenase	-	Naegleria gruberi

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	-	Naegleria gruberi

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
10	35	activity range, profile overview	Naegleria gruberi

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Naegleria gruberi

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	10	activity range, profile overview	Naegleria gruberi

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Naegleria gruberi