Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.1.12 extracted from

  • Guo, L.; Ma, F.; Wei, F.; Fanella, B.; Allen, D.K.; Wang, X.
    Cytosolic phosphorylating glyceraldehyde-3-phosphate dehydrogenases affect Arabidopsis cellular metabolism and promote seed oil accumulation (2014), Plant Cell, 26, 3023-3035 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.12

Protein Variants

Protein Variants Comment Organism
additional information knockout or overexpression of GAPC isozymes in Arabidopsis thaliana causing significant changes in the level of intermediates in the glycolytic pathway and the ratios of ATP/ADP and NAD(P)H/NAD(P). Construction of two double knockout seeds by T-DNA insertion showing over 3% of dry weight decrease in oil content compared with that of the wild-type. In transgenic seeds under the constitutive 35S promoter, oil content is increased up to 42% of dry weight compared with 36% in the wild-type and the fatty acid composition is altered. The transgenic lines exhibit decreased fertility. Seed-specific overexpression lines show over 3% increase in seed oil without compromised seed yield or fecundity, phenotypes, overview Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Arabidopsis thaliana 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Arabidopsis thaliana
-
 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Arabidopsis thaliana Col-0
-
 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P25858
-
-
Arabidopsis thaliana Q9FX54
-
-
Arabidopsis thaliana Col-0 P25858
-
-
Arabidopsis thaliana Col-0 Q9FX54
-
-

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Arabidopsis thaliana 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Arabidopsis thaliana Col-0 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
GAPC
-
 Arabidopsis thaliana
GapC1
-
 Arabidopsis thaliana
GapC2
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction knockout or overexpression of GAPC isozymes causes significant changes in the level of intermediates in the glycolytic pathway and the ratios of ATP/ADP and NAD(P)H/NAD(P). Two double knockout seeds show over 3% of dry weight decrease in oil content compared with that of the wild-type. In transgenic seeds under the constitutive 35S promoter, oil content is increased up to 42% of dry weight compared with 36% in the wild-type and the fatty acid composition is altered. The transgenic lines exhibit decreased fertility. Seed-specific overexpression lines show over 3% increase in seed oil without compromised seed yield or fecundity, phenotypes, overview Arabidopsis thaliana
metabolism the enzyme is involved in glycolysis catalyzing a key reaction Arabidopsis thaliana
physiological function two cytosolic GAPC isozymes play important roles in cellular metabolism and seed oil accumulation. GAPC levels play important roles in the overall cellular production of reductants, energy, and carbohydrate metabolites, and GAPC levels are directly correlated with seed oil accumulation Arabidopsis thaliana