Application | Comment | Organism |
---|---|---|
medicine | glyceraldehyde 3-phosphate dehydrogenase from malignant sources shows altered properties compared with enzyme from healthy subjects | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | inhibition is less pronounced for enzyme from sarcoma tissue as compared to normal muscle tissue | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
NAD+ | pH 7.3, enzyme isolated of sarcoma tissue | Homo sapiens | |
0.036 | - |
NAD+ | pH 8.0, enzyme isolated of sarcoma tissue | Homo sapiens | |
0.04 | - |
NAD+ | pH 8.8, enzyme isolated of sarcoma tissue | Homo sapiens | |
0.047 | - |
NAD+ | pH 8.8, enzyme isolated of healthy patients | Homo sapiens | |
0.062 | - |
NAD+ | pH 8.0, enzyme isolated of healthy patients | Homo sapiens | |
0.071 | - |
NAD+ | pH 7.3, enzyme isolated of healthy patients | Homo sapiens | |
0.087 | - |
D-glyceraldehyde 3-phosphate | pH 7.3, enzyme isolated of sarcoma tissue | Homo sapiens | |
0.089 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, enzyme isolated of sarcoma tissue | Homo sapiens | |
0.11 | - |
D-glyceraldehyde 3-phosphate | pH 8.8, enzyme isolated of sarcoma tissue | Homo sapiens | |
0.149 | - |
D-glyceraldehyde 3-phosphate | pH 7.3, enzyme isolated of healthy patients | Homo sapiens | |
0.159 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, enzyme isolated of healthy patients | Homo sapiens | |
0.16 | - |
D-glyceraldehyde 3-phosphate | pH 8.8, enzyme isolated of healthy patients | Homo sapiens | |
4 | - |
phosphate | pH 8.8, enzyme isolated of healthy patients | Homo sapiens | |
9.9 | - |
phosphate | pH 8.8, enzyme isolated of sarcoma tissue | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
from normal leukocytes of healthy subjects, leukocytes of chronic myeloid leukemia patients and from sarcoma tissue | Homo sapiens |
Storage Stability | Organism |
---|---|
at -20°C storage after dialysis following the first (NH4)2SO4 fractionation step during purification, normal muscle and leukocyte enzyme is stable. Enzyme from chronic myeloid leukemia patients and sarcoma tissue loses about 80% of activity. | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Homo sapiens | 3-phospho-D-glyceroyl phosphate + NADH | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | 8.8 | enzyme isolated from sarcoma tissue, broad | Homo sapiens |
8.6 | - |
enzyme isolated from normal tissue, sharp optimum | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
above, sharp decline in activity for enzyme of patients with chronic myeloid leukemia, no similar sharp decline for enzyme of healthy subjects | Homo sapiens |