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Literature summary for 1.2.1.12 extracted from

  • Holtgrefe, S.; Gohlke, J.; Starmann, J.; Druce, S.; Klocke, S.; Altmann, B.; Wojtera, J.; Lindermayr, C.; Scheibe, R.
    Regulation of plant cytosolic glyceraldehyde 3-phosphate dehydrogenase isoforms by thiol modifications (2008), Physiol. Plant., 133, 211-228.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
C159S the mutant C159S of the isozyme GapC2 shows decreased specific activity Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
glutathione inactivation with 10 mM glutathione is reversible upon addition of 20 mM dithiothreitol Arabidopsis thaliana
glutathione inactivation with 10 mM glutathione is reversible upon addition of 20 mM dithiothreitol Oryctolagus cuniculus
glutathione inactivation with 10 mM glutathione is reversible upon addition of 20 mM dithiothreitol Saccharomyces cerevisiae
glutathione inactivation with 10 mM glutathione is reversible upon addition of 20 mM dithiothreitol Spinacia oleracea
oxidized glutathione inactivation, at least partially reversible upon addition of dithiothreitol. Both residues C155 and C159 are found glutathionylated; inactivation, at least partially reversible upon addition of dithiothreitol. Both residues C155 and C159 are found glutathionylated Arabidopsis thaliana
oxidized glutathione inactivation, at least partially reversible upon addition of dithiothreitol Oryctolagus cuniculus
oxidized glutathione inactivation, at least partially reversible upon addition of dithiothreitol Spinacia oleracea
S-nitrosoglutathione inactivation, at least partially reversible upon addition of dithiothreitol. Both residues C155 and C159 are found nitrosylated; inactivation, at least partially reversible upon addition of dithiothreitol. Both residues C155 and C159 are found nitrosylated; inactivation with 0.5 mM S-nitrosoglutathione is reversible upon addition of 20 mM dithiothreitol Arabidopsis thaliana
S-nitrosoglutathione inactivation, at least partially reversible upon addition of dithiothreitol; inactivation with 0.5 mM S-nitrosoglutathione is reversible upon addition of 20 mM dithiothreitol Oryctolagus cuniculus
S-nitrosoglutathione inactivation with 0.5 mM S-nitrosoglutathione is reversible upon addition of 20 mM dithiothreitol Saccharomyces cerevisiae
S-nitrosoglutathione inactivation, at least partially reversible upon addition of dithiothreitol; inactivation with 0.5 mM S-nitrosoglutathione is reversible upon addition of 20 mM dithiothreitol Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Saccharomyces cerevisiae 5829
-
cytosol
-
Oryctolagus cuniculus 5829
-
cytosol
-
Spinacia oleracea 5829
-
cytosol
-
Arabidopsis thaliana 5829
-
nucleus
-
Arabidopsis thaliana 5634
-
nucleus of protoplast, both isoforms GapC1 and GapC2 Arabidopsis thaliana 5634
-
protoplast
-
Arabidopsis thaliana
-
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P25858 ecotype Columbia
-
Arabidopsis thaliana P25858 isoform GapC1
-
Arabidopsis thaliana Q56WJ4 isoform GapC2
-
Oryctolagus cuniculus
-
-
-
Saccharomyces cerevisiae
-
-
-
Spinacia oleracea
-
-
-

Oxidation Stability

Oxidation Stability Organism
stable after reactivation and desalting for at least 2 h when incubated in the presence of 20 mM dithiothreitol, but less stable when both, NAD+ and the reductant, are omitted in the pre-incubation assays, under this condition the enzymes seem to be very sensitive towards traces of oxygen in the media, but upon addition of 20 mM dithiothritol after 1 h, the full activity is restored Saccharomyces cerevisiae
stable after reactivation and desalting for at least 2 h when incubated in the presence of 20 mM dithiothreitol, but less stable when both, NAD+ and the reductant, are omitted in the pre-incubation assays, under this condition the enzymes seem to be very sensitive towards traces of oxygen in the media, but upon addition of 20 mM dithiothritol after 1 h, the full activity is restored Oryctolagus cuniculus
stable after reactivation and desalting for at least 2 h when incubated in the presence of 20 mM dithiothreitol, but less stable when both, NAD+ and the reductant, are omitted in the pre-incubation assays, under this condition the enzymes seem to be very sensitive towards traces of oxygen in the media, but upon addition of 20 mM dithiothritol after 1 h, the full activity is restored Spinacia oleracea
stable after reactivation and desalting for at least 2 h when incubated in the presence of 20 mM dithiothreitol, but less stable when both, NAD+ and the reductant, are omitted in the pre-incubation assays, under this condition the enzymes seem to be very sensitive towards traces of oxygen in the media, but upon addition of 20 mM dithiothritol after 1 h, the full activity is restored Arabidopsis thaliana

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Q-Sepharose column chromatography Saccharomyces cerevisiae
ammonium sulfate precipitation and Q-Sepharose column chromatography Oryctolagus cuniculus
ammonium sulfate precipitation and Q-Sepharose column chromatography Spinacia oleracea
ammonium sulfate precipitation and Q-Sepharose column chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Arabidopsis thaliana
-
leaf
-
Spinacia oleracea
-
leaf
-
Arabidopsis thaliana
-
muscle
-
Oryctolagus cuniculus
-
silique
-
Arabidopsis thaliana
-
stem
-
Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30
-
purified isozyme GapC1 Arabidopsis thaliana
33
-
purified GapC2 C159S mutant isozyme Arabidopsis thaliana
60
-
purified isozyme GapC2 Arabidopsis thaliana
90
-
purified enzyme Spinacia oleracea
100
-
purified enzyme Saccharomyces cerevisiae
100
-
purified enzyme Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Saccharomyces cerevisiae 3-phospho-D-glyceroyl phosphate + NADH + H+
-
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Oryctolagus cuniculus 3-phospho-D-glyceroyl phosphate + NADH + H+
-
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Spinacia oleracea 3-phospho-D-glyceroyl phosphate + NADH + H+
-
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Arabidopsis thaliana 3-phospho-D-glyceroyl phosphate + NADH + H+
-
?

Subunits

Subunits Comment Organism
More enzyme can bind top a partial gene sequence of NADP-dependent malated dehydrogenase EC 1.2.1.37 Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
cytosolic NAD-dependent glyceraldehyde 3-P dehydrogenase
-
Saccharomyces cerevisiae
cytosolic NAD-dependent glyceraldehyde 3-P dehydrogenase
-
Oryctolagus cuniculus
cytosolic NAD-dependent glyceraldehyde 3-P dehydrogenase
-
Spinacia oleracea
cytosolic NAD-dependent glyceraldehyde 3-P dehydrogenase
-
Arabidopsis thaliana
GapC1 isozyme Arabidopsis thaliana
GapC2 isozyme Arabidopsis thaliana
GAPDH
-
Saccharomyces cerevisiae
GAPDH
-
Oryctolagus cuniculus
GAPDH
-
Spinacia oleracea
GAPDH
-
Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Saccharomyces cerevisiae
NAD+
-
Oryctolagus cuniculus
NAD+
-
Spinacia oleracea
NAD+
-
Arabidopsis thaliana