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Literature summary for 1.2.1.12 extracted from

  • Alvarez, A.H.; Martinez-Cadena, G.; Silva, M.E.; Saavedra, E.; Avila, E.E.
    Entamoeba histolytica: ADP-ribosylation of secreted glyceraldehyde-3-phosphate dehydrogenase (2007), Exp. Parasitol., 117, 349-356.
    View publication on PubMed
Search for more literature for 1.2.1.12

Activating Compound

Activating Compound Comment Organism Structure
NAD+ highest activity in the presence of 0.2 mM NAD+ at 30°C Entamoeba histolytica

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Entamoeba histolytica 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 ADP-ribosylated enzyme subunit, SDS-PAGE Entamoeba histolytica

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica Q27646 fragment
-
Entamoeba histolytica HM1 Q27646 fragment
-

Posttranslational Modification

Posttranslational Modification Comment Organism
ribosylation mono-ADP-ribosylation does not affect GADPH activity Entamoeba histolytica

Source Tissue

Source Tissue Comment Organism Textmining
trophozoite
-
 Entamoeba histolytica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.1767
-
 crude extract, at 30 °C Entamoeba histolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Entamoeba histolytica 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Entamoeba histolytica HM1 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
GAPDH
-
 Entamoeba histolytica

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Entamoeba histolytica

pI Value

Organism Comment pI Value Maximum pI Value
Entamoeba histolytica isoelectric focusing
-
 6.4