Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.1.12 extracted from

  • Cardoso, C.L.; de Moraes, M.C.; Guido, R.V.; Oliva, G.; Andricopulo, A.D.; Wainer, I.W.; Cass, Q.B.
    The development of an immobilized enzyme reactor containing glyceraldehyde-3-phosphate dehydrogenase from Trypanosoma cruzi: the effect of species specific differences on the immobilization (2008), Analyst, 133, 93-99.
    View publication on PubMed
Search for more literature for 1.2.1.12

Cloned(Commentary)

Cloned (Comment) Organism
-
 Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.258
-
 NAD+ free enzyme Trypanosoma cruzi
0.425
-
 D-Glyceraldehyde-3-phosphate free enzyme Trypanosoma cruzi
0.5
-
 D-Glyceraldehyde-3-phosphate GAPDH immobilized on an octyl silica column Trypanosoma cruzi
0.674
-
 NAD+ GAPDH immobilized on an octyl silica column Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Trypanosoma cruzi

Storage Stability

Storage Stability Organism
-80°C, triethanolamine (100 mM, pH 7.5), containing 1.0 mM beta-mercaptoethanol, 1.0 mM EDTA, 30 mM sodium arsenate heptahydrate, 1.0 mM phenylmethylsulfonyl fluoride, 50 mM NAD+, 1.0 mM pepstatin, 1.0 mM leupeptin, 0.5 M KCl, and glycerol 10% (v/v), 8 months, 1% loss of activity Trypanosoma cruzi
25°C, free enzyme in Tris-HCl (50 mM, pH 8.6), containing 1.0 mM beta-mercaptoethanol, 30 mM sodium arsenate heptahydrate, and 1.0 mM EDTA, 99% of the enzymatic activity is retained after 24 h and 57% after 120 h Trypanosoma cruzi
4°C, free enzyme in buffer 8, 55% of the enzymatic activity is retained after dialysis and after 48 h the calculated activity has fallen to 7% Trypanosoma cruzi
4°C, free enzyme in Tris-HCl (50 mM, pH 8.6), containing 1.0 mM beta-mercaptoethanol, 30 mM sodium arsenate heptahydrate, and 1.0 mM EDTA, 55% of the enzymatic activity is retained after dialysis and after 48 h the calculated activity has fallen to 25% Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Trypanosoma cruzi 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
GAPDH
-
 Trypanosoma cruzi

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Trypanosoma cruzi