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Literature summary for 1.2.1.12 extracted from

  • Wiggers, H.J.; Cheleski, J.; Zottis, A.; Oliva, G.; Andricopulo, A.D.; Montanari, C.A.
    Effects of organic solvents on the enzyme activity of Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase in calorimetric assays (2007), Anal. Biochem., 370, 107-114.
    View publication on PubMed
Search for more literature for 1.2.1.12

Activating Compound

Activating Compound Comment Organism Structure
DMSO activity is increased about 2fold in the presence of 5% DMSO (v/v) Trypanosoma cruzi
methanol activity is increased about 6fold in the presence of 5% methanol (v/v) Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0102
-
 D-glyceraldehyde 3-phosphate in the presence of 5% (v/v) methanol, in TEA-HCl buffer (pH 7.5) at 25°C Trypanosoma cruzi
0.0102
-
 D-glyceraldehyde 3-phosphate in the presence of 5% (v/v) methanol Trypanosoma cruzi
0.0108
-
 D-glyceraldehyde 3-phosphate in the presence of 5% (v/v) DMSO, in TEA-HCl buffer (pH 7.5) at 25°C Trypanosoma cruzi
0.0108
-
 D-glyceraldehyde 3-phosphate in the presence of 5% (v/v) DMSO Trypanosoma cruzi
0.0393
-
 D-glyceraldehyde 3-phosphate in the absence of DMSO and methanol, in TEA-HCl buffer (pH 7.5) at 25°C Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Trypanosoma cruzi 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 r

Synonyms

Synonyms Comment Organism
GAPDH
-
 Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
71.4
-
 D-glyceraldehyde 3-phosphate in the absence of DMSO and methanol, in TEA-HCl buffer (pH 7.5) at 25°C Trypanosoma cruzi
118
-
 D-glyceraldehyde 3-phosphate in the presence of 5% (v/v) DMSO, in TEA-HCl buffer (pH 7.5) at 25°C Trypanosoma cruzi
442
-
 D-glyceraldehyde 3-phosphate in the presence of 5% (v/v) methanol, in TEA-HCl buffer (pH 7.5) at 25°C Trypanosoma cruzi

Cofactor

Cofactor Comment Organism Structure
NAD+ dependent Trypanosoma cruzi