Crystallization (Comment) | Organism |
---|---|
holoenzyme and apoenzyme | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
4 * 36000, SDS-PAGE | Thermus aquaticus |
36000 | - |
4 * 36000, SDS-PAGE | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Oryctolagus cuniculus | - |
- |
- |
Thermus aquaticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermus aquaticus |
- |
Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16 | - |
apoenzyme | Thermus aquaticus |
16.5 | - |
holoenzyme | Thermus aquaticus |
26.69 | - |
- |
Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glyceroyl phosphate + NADH | - |
Oryctolagus cuniculus | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
r | |
3-phospho-D-glyceroyl phosphate + NADH | - |
Geobacillus stearothermophilus | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
r | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Thermus aquaticus | 3-phospho-D-glyceroyl phosphate + NADH | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Oryctolagus cuniculus | 3-phospho-D-glyceroyl phosphate + NADH | - |
r | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Geobacillus stearothermophilus | 3-phospho-D-glyceroyl phosphate + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Oryctolagus cuniculus |
tetramer | 4 * 36000, SDS-PAGE | Thermus aquaticus |
tetramer | 4 * 36000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
58 | - |
pH 7.0, 20 min, 50% inactivation | Oryctolagus cuniculus |
75 | - |
pH 7.0, 20 min, 50% inactivation | Geobacillus stearothermophilus |
100 | - |
pH 7.0, 20 min, 50% inactivation | Thermus aquaticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermus aquaticus | |
NAD+ | - |
Oryctolagus cuniculus | |
NAD+ | enzyme contains 4 equivalents of firmly bound NAD+ | Geobacillus stearothermophilus | |
NADH | cofactor | Oryctolagus cuniculus | |
NADH | cofactor | Geobacillus stearothermophilus |