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Literature summary for 1.2.1.12 extracted from
- Characterization of two glyceraldehyde-3-phosphate dehydrogenase isoenzymes from the pentalenolactone producer Streptomyces arenae (1983), J. Bacteriol., 153, 930-936.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| pentalenolactone | pentalenolactone-sensitive enzyme is strongly inhibited, pentalenolactone-insensitive enzyme is not inhibited | Streptomyces arenae |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
D-glyceraldehyde 3-phosphate | pentalenolactone-insensitive enzyme | Streptomyces arenae | |
| 0.11 | - |
NAD+ | pentalenolactone-sensitive enzyme | Streptomyces arenae | |
| 0.12 | - |
NAD+ | pentalenolactone-insensitive enzyme | Streptomyces arenae | |
| 0.25 | - |
D-glyceraldehyde 3-phosphate | pentalenolactone-sensitive enzyme | Streptomyces arenae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
4 * 43000, pentalenolactone-sensitive enzyme, gel filtration | Streptomyces arenae |
| 43000 | - |
8 * 43000, pentalenolactone-insensitive enzyme, gel filtration | Streptomyces arenae |
| 180000 | - |
gel filtration, pentalenolactone-sensitive enzyme | Streptomyces arenae |
| 290000 | - |
gel filtration, pentalenolactone-insensitive enzyme | Streptomyces arenae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces arenae | - |
TÜ469, contains pentalenolactone-insensitive glyceraldehyde-3-phosphate dehydrogenase under conditions of production of pentalenolactone. In complex medium no pentalenolactone production is observed and a pentalenolactone-sensitive D-glyceraldehyde 3-phosphate dehydrogenase, rather than the insensitive enzyme can be detected | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces arenae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 33 | - |
pentalenolactone-insensitive enzyme | Streptomyces arenae |
| 112 | - |
pentalenolactone-sensitive enzyme | Streptomyces arenae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, pentalenolactone-insensitive enzyme stable up to 6 months. Pentalenolactone-sensitive enzyme loses activity with a half-life of 7 days | Streptomyces arenae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-phospho-D-glyceroyl phosphate + NADH | - |
Streptomyces arenae | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
r | |
| D-glyceraldehyde 3-phosphate + arsenate + NAD+ | - |
Streptomyces arenae | 3-phospho-D-glyceroyl arsenate + NADH | - |
? | |
| D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Streptomyces arenae | 3-phospho-D-glyceroyl phosphate + NADH | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 43000, pentalenolactone-insensitive enzyme, gel filtration | Streptomyces arenae |
| tetramer | 4 * 43000, pentalenolactone-sensitive enzyme, gel filtration | Streptomyces arenae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Streptomyces arenae | |
| NADH | cofactor | Streptomyces arenae | |
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