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Literature summary for 1.2.1.10 extracted from

  • Lo, J.; Zheng, T.; Hon, S.; Olson, D.G.; Lynd, L.R.
    The bifunctional alcohol and aldehyde dehydrogenase gene, adhE, is necessary for ethanol production in Clostridium thermocellum and Thermoanaerobacterium saccharolyticum (2015), J. Bacteriol., 197, 1386-1393.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.10

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus A0A0H3W5U9 bifunctional aldehyde-alcohol dehydrogenase
-
Acetivibrio thermocellus A3DCI2
-
-
Thermoanaerobacterium saccharolyticum A0A0H3W5K4 bifunctional aldehyde-alcohol dehydrogenase
-
Thermoanaerobacterium saccharolyticum I3VSF1
-
-
Thermoanaerobacterium saccharolyticum DSM 8691 I3VSF1
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.04
-
 gene AdhE deletion mutant, cosubstrate NADH, pH 7.0, 55°C Acetivibrio thermocellus
0.05
-
 wild-type, cosubstrate NADPH, pH 7.0, 55°C Thermoanaerobacterium saccharolyticum
0.09
-
 gene AdhE deletion mutant, cosubstrate NADH, pH 7.0, 55°C Thermoanaerobacterium saccharolyticum
0.09
-
 gene AdhE deletion mutant, cosubstrate NADPH, pH 7.0, 55°C Thermoanaerobacterium saccharolyticum
0.09
-
 wild-type, cosubstrate NADH, pH 7.0, 55°C Thermoanaerobacterium saccharolyticum
0.1
-
 gene AdhE deletion mutant, cosubstrate NADPH, pH 7.0, 55°C Acetivibrio thermocellus
0.16
-
 wild-type, cosubstrate NADPH, pH 7.0, 55°C Acetivibrio thermocellus
2.18
-
 wild-type, cosubstrate NADH, pH 7.0, 55°C Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + NADH + H+
-
 Acetivibrio thermocellus acetaldehyde + CoA + NAD+
-
 ?
acetyl-CoA + NADH + H+
-
 Thermoanaerobacterium saccharolyticum acetaldehyde + CoA + NAD+
-
 ?
acetyl-CoA + NADH + H+
-
 Acetivibrio thermocellus DSM 1237 acetaldehyde + CoA + NAD+
-
 ?
acetyl-CoA + NADH + H+
-
 Thermoanaerobacterium saccharolyticum DSM 8691 acetaldehyde + CoA + NAD+
-
 ?
acetyl-CoA + NADPH + H+
-
 Acetivibrio thermocellus acetaldehyde + CoA + NADP+
-
 ?
acetyl-CoA + NADPH + H+
-
 Thermoanaerobacterium saccharolyticum acetaldehyde + CoA + NADP+
-
 ?
acetyl-CoA + NADPH + H+
-
 Acetivibrio thermocellus DSM 1237 acetaldehyde + CoA + NADP+
-
 ?
acetyl-CoA + NADPH + H+
-
 Thermoanaerobacterium saccharolyticum DSM 8691 acetaldehyde + CoA + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
AdhE
-
 Acetivibrio thermocellus
AdhE
-
 Thermoanaerobacterium saccharolyticum
AdhE bifunctional alcohol and aldehyde dehydrogenase gene Acetivibrio thermocellus
AdhE bifunctional alcohol and aldehyde dehydrogenase gene Thermoanaerobacterium saccharolyticum
Tsac_0416
-
 Thermoanaerobacterium saccharolyticum

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Acetivibrio thermocellus
NADH
-
 Thermoanaerobacterium saccharolyticum
NADPH
-
 Acetivibrio thermocellus
NADPH
-
 Thermoanaerobacterium saccharolyticum

General Information

General Information Comment Organism
physiological function deletion of the bifunctional alcohol and aldehyde dehydrogenase gene adhE reduces ethanol production by more than 95%. In the deletion strain, fermentation products shift from ethanol to lactate production and result in lower cell density and longer time to reach maximal cell density. It loses more than 85% of alcohol dehydrogenase activity. Aldehyde dehydrogenase activity does not appear to be affected, although its activity is low in cell extracts. Adding ubiquinone-0 to the aldehyde dehydrogenase assay increases activity in the parent strain but does not increase activity in the adhE deletion strain Thermoanaerobacterium saccharolyticum
physiological function deletion of the bifunctional alcohol and aldehyde dehydrogenase gene adhE reduces ethanol production by more than 95%. In the deletion strains, fermentation products shift from ethanol to lactate production and result in lower cell density and longer time to reach maximal cell density. The deletion strain additionally contains a point mutation in the lactate dehydrogenase gene, which appears to deregulate its activation by fructose 1,6-bisphosphate, leading to constitutive activation of lactate dehydrogenase Acetivibrio thermocellus
physiological function deletion of adhE reduces ethanol production by more than 95%. Fermentation products shift from ethanol to lactate production and result in lower cell density and longer time to reach maximal cell density. The adhE deletion strain loses more than 85% of alcohol dehydrogenase activity. Aldehyde dehydrogenase activity does not appear to be affected Thermoanaerobacterium saccharolyticum
physiological function deletion of adhE reduces ethanol production by more than 95%. Fermentation products shift from ethanol to lactate production and result in lower cell density and longer time to reach maximal cell density. The adhE deletion strain loses more than 90% of ALDH and ADH activity in cell extracts Acetivibrio thermocellus