Cloned (Comment) | Organism |
---|---|
- |
Paraburkholderia xenovorans |
Protein Variants | Comment | Organism |
---|---|---|
C131A | inactive | Paraburkholderia xenovorans |
C131S | inactive | Paraburkholderia xenovorans |
D208A | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Paraburkholderia xenovorans |
I171A | level of activation of BphI by the mutant enzyme are reduced by more than 3fold in the presence of NADH and more than 4.5fold when the enzyme is undergoing turnover. The mutation results in a 35% reduction in acetaldehyde channeling efficiency | Paraburkholderia xenovorans |
I171F | the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme | Paraburkholderia xenovorans |
I195A | the variant has a 20fold higher catalytic efficiency for butyraldehyde and pentaldehyde compared to the catalytic efficiency of the wild type toward its natural substrate acetaldehyde. The mutation results in a 35% reduction in acetaldehyde channeling efficiency | Paraburkholderia xenovorans |
I195F | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Paraburkholderia xenovorans |
I195W | the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme | Paraburkholderia xenovorans |
N170A | the mutation does not substantially alter aldehyde channeling efficiencies. The level of activation of BphI by the mutant enzyme are reduced by more than 3fold in the presence of NADH and more than 4.5fold when the enzyme is undergoing turnover | Paraburkholderia xenovorans |
N170D | level of activation of BphI by the mutant enzyme are reduced by more than 3fold in the presence of NADH and more than 4.5fold when the enzyme is undergoing turnover | Paraburkholderia xenovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.2 | - |
picolinaldehyde | mutant enzyme I171A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
4.6 | - |
Butyraldehyde | mutant enzyme I195A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
6.9 | - |
picolinaldehyde | mutant enzyme I195A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
8.2 | - |
pentaldehyde | mutant enzyme I195A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
12 | - |
picolinaldehyde | mutant enzyme I171F, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
12.6 | - |
Butyraldehyde | mutant enzyme I171A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
17 | - |
acetaldehyde | mutant enzyme I171A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
18.2 | - |
picolinaldehyde | wild type enzyme, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
18.7 | - |
acetaldehyde | mutant enzyme D208A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
19.3 | - |
Butyraldehyde | mutant enzyme I171F, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
20.4 | - |
propionaldehyde | mutant enzyme I171A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
23.1 | - |
propionaldehyde | wild type enzyme, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
23.6 | - |
acetaldehyde | wild type enzyme, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
27.2 | - |
propionaldehyde | mutant enzyme I171F, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
31.5 | - |
propionaldehyde | mutant enzyme I195A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
31.7 | - |
Butyraldehyde | wild type enzyme, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
34.9 | - |
acetaldehyde | mutant enzyme I171F, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
45.6 | - |
acetaldehyde | mutant enzyme I195F, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
79.5 | - |
propionaldehyde | mutant enzyme I195F, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
133 | - |
acetaldehyde | mutant enzyme I195A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + CoA + NAD+ | Paraburkholderia xenovorans | - |
acetyl-CoA + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paraburkholderia xenovorans | Q79AF6 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Paraburkholderia xenovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + CoA + NAD+ | - |
Paraburkholderia xenovorans | acetyl-CoA + NADH + H+ | - |
? | |
acetaldehyde + CoA + NAD+ | - |
Paraburkholderia xenovorans | acetyl-CoA + NADH + H+ | - |
r | |
butyraldehyde + CoA + NAD+ | - |
Paraburkholderia xenovorans | butyryl-CoA + NADH + H+ | - |
r | |
additional information | BphJ forms a heterotetrameric complex with the class II aldolase BphI that channels aldehydes produced in the aldol cleavage reaction to the dehydrogenase via a molecular tunnel | Paraburkholderia xenovorans | ? | - |
? | |
pentaldehyde + CoA + NAD+ | - |
Paraburkholderia xenovorans | pentyl-CoA + NADH + H+ | - |
r | |
picolinaldehyde + CoA + NAD+ | - |
Paraburkholderia xenovorans | picolinyl-CoA + NADH + H+ | - |
r | |
propionaldehyde + CoA + NAD+ | - |
Paraburkholderia xenovorans | propionyl-CoA + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
ACDH | - |
Paraburkholderia xenovorans |
BphJ | - |
Paraburkholderia xenovorans |
nonphosphorylating acylating aldehyde dehydrogenase | - |
Paraburkholderia xenovorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
picolinaldehyde | mutant enzyme I171A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
2.4 | - |
picolinaldehyde | mutant enzyme I171F, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
2.8 | - |
picolinaldehyde | wild type enzyme, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
3.2 | - |
picolinaldehyde | mutant enzyme I195A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
3.4 | - |
propionaldehyde | mutant enzyme I195W, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
6 | - |
propionaldehyde | mutant enzyme I171F, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
6.9 | - |
acetaldehyde | mutant enzyme I195W, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
7.1 | - |
Butyraldehyde | mutant enzyme I171F, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
7.6 | - |
acetaldehyde | mutant enzyme D208A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
7.6 | - |
propionaldehyde | mutant enzyme I195F, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
7.8 | - |
acetaldehyde | mutant enzyme I171F, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
9.5 | - |
Butyraldehyde | wild type enzyme, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
12.1 | - |
propionaldehyde | mutant enzyme I195A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
12.6 | - |
Butyraldehyde | mutant enzyme I195A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
13 | - |
pentaldehyde | mutant enzyme I195A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
15.4 | - |
acetaldehyde | mutant enzyme I195F, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
16.3 | - |
propionaldehyde | wild type enzyme, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
16.7 | - |
Butyraldehyde | mutant enzyme I171A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
17.2 | - |
acetaldehyde | wild type enzyme, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
19.7 | - |
acetaldehyde | mutant enzyme I195A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
24.9 | - |
propionaldehyde | mutant enzyme I171A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
37.9 | - |
acetaldehyde | mutant enzyme I171A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Paraburkholderia xenovorans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00011 | - |
picolinaldehyde | mutant enzyme I195W, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
0.00076 | - |
picolinaldehyde | mutant enzyme I195F, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
0.0265 | - |
pentaldehyde | mutant enzyme I195W, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
0.0346 | - |
pentaldehyde | mutant enzyme I195F, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
0.0387 | - |
picolinaldehyde | mutant enzyme I171F, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
0.0425 | - |
propionaldehyde | mutant enzyme I195W, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
0.0433 | - |
Butyraldehyde | mutant enzyme I195F, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
0.0458 | - |
Butyraldehyde | mutant enzyme I195W, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
0.0728 | - |
pentaldehyde | mutant enzyme I171A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
0.076 | - |
pentaldehyde | wild type enzyme, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
0.147 | - |
acetaldehyde | mutant enzyme I195A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
0.152 | - |
acetaldehyde | mutant enzyme I195W, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
0.154 | - |
picolinaldehyde | wild type enzyme, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
0.167 | - |
propionaldehyde | mutant enzyme I195F, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
0.221 | - |
propionaldehyde | mutant enzyme I171F, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
0.223 | - |
acetaldehyde | mutant enzyme I171F, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
0.3 | - |
Butyraldehyde | wild type enzyme, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
0.37 | - |
Butyraldehyde | mutant enzyme I171F, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
0.384 | - |
propionaldehyde | mutant enzyme I195A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
0.457 | - |
acetaldehyde | mutant enzyme I195F, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
0.46 | - |
acetaldehyde | mutant enzyme D208A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
0.468 | - |
picolinaldehyde | mutant enzyme I195A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
0.597 | - |
picolinaldehyde | mutant enzyme I171A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
0.7 | - |
propionaldehyde | wild type enzyme, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
0.73 | - |
acetaldehyde | wild type enzyme, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
1.219 | - |
propionaldehyde | mutant enzyme I171A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
1.323 | - |
Butyraldehyde | mutant enzyme I171A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
1.584 | - |
pentaldehyde | mutant enzyme I195A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
2.224 | - |
acetaldehyde | mutant enzyme I171A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans | |
2.763 | - |
Butyraldehyde | mutant enzyme I195A, at 25°C in 100 mM HEPES buffer (pH 8.0) | Paraburkholderia xenovorans |