Cloned (Comment) | Organism |
---|---|
gene adhE, pyruvate-formate-lyase-deactivase with alcohol and acetaldehyde dehydrogenase activity | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 3fold activation with 0.00003 mM, 5fold activation with 0.03 mM, activation only of NADH oxidation, not NAD+ reduction | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
96000 | - |
ca. 40 * 96000, pyruvate-formate-lyase-deactivase with alcohol and aldehyde dehydrogenase activity, nucleotide sequence | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + CoA + NAD+ | Escherichia coli | - |
acetyl-CoA + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
grown anaerobically | - |
Purification (Comment) | Organism |
---|---|
from anaerobically grown cells, rod-shaped | Escherichia coli |
Storage Stability | Organism |
---|---|
-60°C, 20 mM MOPS-KOH pH 7.6, 0.1 mM EDTA, up to one year | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + CoA + NAD+ | - |
Escherichia coli | acetyl-CoA + NADH | - |
? | |
acetaldehyde + CoA + NAD+ | - |
Escherichia coli | acetyl-CoA + NADH | - |
r | |
acetyl-CoA + NADH | - |
Escherichia coli | acetaldehyde + CoA + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
polymer | ca. 40 * 96000, pyruvate-formate-lyase-deactivase with alcohol and aldehyde dehydrogenase activity, nucleotide sequence | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
CoA | - |
Escherichia coli | |
NAD+ | - |
Escherichia coli |