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Literature summary for 1.18.1.3 extracted from

  • Umeda, T.; Katsuki, J.; Ashikawa, Y.; Usami, Y.; Inoue, K.; Noguchi, H.; Fujimoto, Z.; Yamane, H.; Nojiri, H.
    Crystallization and preliminary X-ray diffraction studies of a ferredoxin reductase component of carbazole 1,9a-dioxygenase from Novosphingobium sp. KA1 (2010), Acta Crystallogr. Sect. F, 66, 712-714.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene carAd or fdr, from genomic DNA, expression of His-tagged Red in Escherichia coli strain BL21(DE3) Novosphingobium sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
recombinant His-tagged RedIIA from KA1, hanging-drop vapour-diffusion, 5°C, method using PEG 4000, X-ray diffraction structure determination and analysis at 1.58 A resolution, modelling Novosphingobium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39800
-
x * 39800, recombinant His-tagged RedIIA, SDS-PAGE Novosphingobium sp.

Organism

Organism UniProt Comment Textmining
Novosphingobium sp.
-
carAd or fdr gene
-
Novosphingobium sp. KA1
-
carAd or fdr gene
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged RedIIA from Escherichia coli strain BL21(DE3) by metal affinity chromatography and gel filtration Novosphingobium sp.

Subunits

Subunits Comment Organism
? x * 39800, recombinant His-tagged RedIIA, SDS-PAGE Novosphingobium sp.

Synonyms

Synonyms Comment Organism
ferredoxin-NADH oxidoreductase
-
Novosphingobium sp.
Red
-
Novosphingobium sp.
RedIIA
-
Novosphingobium sp.

Cofactor

Cofactor Comment Organism Structure
Ferredoxin
-
Novosphingobium sp.
NADH
-
Novosphingobium sp.

General Information

General Information Comment Organism
additional information the ferredoxin-NADH oxidoreductase Red is part of the carbazole 1,9a-dioxygenase. Red and is classified as a class IIA Rieske oxygenase Novosphingobium sp.