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Literature summary for 1.18.1.3 extracted from

  • Haigler, B.E.; Gibson, D.T.
    Purification and properties of NADH-ferredoxinNAP reductase, a component of naphthalene dioxygenase from Pseudomonas sp. strain NCIB 9816 (1990), J. Bacteriol., 172, 457-464.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.18.1.3

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 10 mM, 30% inhibition Pseudomonas sp.
iodoacetate 10 mM, 50% inhibition Pseudomonas sp.
NEM 10 mM, 67% inhibition Pseudomonas sp.
PCMB 0.0005 mM, 94% inhibition Pseudomonas sp.
Sodium azide 40 mM, 46% inhibition Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Iron iron-sulfur flavoprotein, protein contains 1.8 gatom of iron and 2.0 gatom of acid-labile sulfur Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34900
-
 non-denaturing PAGE Pseudomonas sp.
36000
-
 1 * 36000, SDS-PAGE Pseudomonas sp.
37000
-
 gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp.
-
 NADH-ferredoxinNAP reductase component of naphthalene dioxygenase
-
Pseudomonas sp. NCIB 9816
-
-
-

Purification (Commentary)

Purification (Comment) Organism
NADH-ferredoxinNAP reductase component of naphthalene dioxygenase Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
397
-
-
 Pseudomonas sp.

Storage Stability

Storage Stability Organism
-20°C, 1 month, minimal loss of activity Pseudomonas sp.
0-5°C, 30% loss of activity after 5 days Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricyanide + NADH
-
 Pseudomonas sp. 2 ferrocyanide + NAD+ + H+
-
 ?
2 ferricyanide + NADH
-
 Pseudomonas sp. NCIB 9816 2 ferrocyanide + NAD+ + H+
-
 ?
2 ferricytochrome c + NADH
-
 Pseudomonas sp. 2 ferrocytochrome c + NAD+ + H+
-
 ?
2 ferricytochrome c + NADH
-
 Pseudomonas sp. NCIB 9816 2 ferrocytochrome c + NAD+ + H+
-
 ?
NADH + oxidized 2,6-dichlorophenolindophenol + H+
-
 Pseudomonas sp. NAD+ + reduced 2,6-dichlorophenolindophenol
-
 ?
NADH + oxidized 2,6-dichlorophenolindophenol + H+
-
 Pseudomonas sp. NCIB 9816 NAD+ + reduced 2,6-dichlorophenolindophenol
-
 ?
NADH + oxidized nitro blue tetrazolium + H+
-
 Pseudomonas sp. NAD+ + reduced nitro blue tetrazolium
-
 ?
NADH + oxidized nitro blue tetrazolium + H+
-
 Pseudomonas sp. NCIB 9816 NAD+ + reduced nitro blue tetrazolium
-
 ?
NADPH + oxidized cytochrome c 39% of the activity with NADH Pseudomonas sp. NADP+ + reduced cytochrome c
-
 ?
NADPH + oxidized cytochrome c 39% of the activity with NADH Pseudomonas sp. NCIB 9816 NADP+ + reduced cytochrome c
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 36000, SDS-PAGE Pseudomonas sp.

Synonyms

Synonyms Comment Organism
NADH-ferredoxinNAP reductase component of naphthalene dioxygenase multicomponent enzyme system Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
0.5
-
 5 d, 30% loss of activity Pseudomonas sp.
21
-
 room temperature, 8 h, 50% loss of activity Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
FAD 1 mol of FAD is bound per mol of enzyme Pseudomonas sp.
NADH
-
 Pseudomonas sp.
NADPH 39% of the activity with NADH in the reaction with cytochrome c Pseudomonas sp.