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Literature summary for 1.17.7.4 extracted from

  • Janthawornpong, K.; Krasutsky, S.; Chaignon, P.; Rohmer, M.; Poulter, C.D.; Seemann, M.
    Inhibition of IspH, a [4Fe-4S]2+ enzyme involved in the biosynthesis of isoprenoids via the methylerythritol phosphate pathway (2013), J. Am. Chem. Soc., 135, 1816-1822.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.17.7.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
(E)-4-amino-3-methylbut-2-en-1-yl diphosphate
-
 Escherichia coli
(E)-4-mercapto-3-methyl-but-2-en-1-yl diphosphate
-
 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatpgraphy Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Escherichia coli isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Synonyms

Synonyms Comment Organism
ispH
-
 Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00002
-
 (E)-4-mercapto-3-methyl-but-2-en-1-yl diphosphate pH 8, 37°C Escherichia coli
0.000054
-
 (E)-4-amino-3-methylbut-2-en-1-yl diphosphate pH 8, 37°C Escherichia coli