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Literature summary for 1.17.1.4 extracted from

  • Sauer, P.; Frebortova, J.; Sebela, M.; Galuszka, P.; Jacobsen, S.; Pec, P.; Frebort, I.
    Xanthine dehydrogenase of pea seedlings: a member of the plant molybdenum oxidoreductase family (2002), Plant Physiol. Biochem., 40, 393-400.
No PubMed abstract available
Search for more literature for 1.17.1.4

Inhibitors

Inhibitors Comment Organism Structure
adenine 0.1 mM, 21% residual activity Pisum sativum
allopurinol 0.1 mM, complete inhibition Pisum sativum
N6-furfuryladenine 0.1 mM, 59% residual activity Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 xanthine pH 8.5 Pisum sativum
0.018
-
 hypoxanthine pH 8.5 Pisum sativum
0.025
-
 NAD+ pH 8.5 Pisum sativum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
 2 * 150000, SDS-PAGE Pisum sativum
300000
-
 gel filtration Pisum sativum

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pisum sativum

Source Tissue

Source Tissue Comment Organism Textmining
seedling etiolated Pisum sativum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17
-
 pH 8.5 Pisum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hypoxanthine + NAD+ + H2O 44% of the activity with xanthine Pisum sativum xanthine + NADH + H+
-
 ?
indole-3-acetaldehyde + NAD+ + H2O and similar aldehydes, 2-3% of the activity with xanthine Pisum sativum indole-3-acetate + NADH + H+
-
 ?
xanthine + NAD+ + H2O
-
 Pisum sativum urate + NADH
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 150000, SDS-PAGE Pisum sativum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Pisum sativum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pisum sativum
NADH
-
 Pisum sativum

pI Value

Organism Comment pI Value Maximum pI Value
Pisum sativum isoelectric focusing
-
 5.3